HTA9 | Probable histone H2A variant 3

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AS10 718 | clonality: polyclonal | host: rabbit | reactivity: Arabidopsis thaliana


28 st
Item No:
AS10 718

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product information

Variant histone H2A may replace conventional H2A in a subset of nucleosomes. Synonymes: H2A.F/Z 3


KLH-conjugated synthetic peptide derived from Arabidopsis thaliana HTA9 UniProt: Q9C944, TAIR: At1g52740

Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum in PBS pH 7.4.
Format Lyophilized
Quantity 50 µg

For reconstitution add 50 ĩl of sterile water.


Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Western blot (WB)
Related products

AS11 1801 | anti-H1 | Histone H1, rabbit antibodies
AS10 710 | anti-H3 | Histone H3, rabbit antibody
AS10 710A | anti-H3 | Histone H3 (affinity purified), rabbit antibodies for ChIP
AS11 1753 | anti-HDT3 | Histone deacetylase, rabbit antibody

collection of antibodies to DNA/RNA/cell cycle

Plant protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution

1 : 1000 (WB)

Expected | apparent MW

14.2 kDa (Arabidopsis thaliana)

Confirmed reactivity

Arabidopsis thaliana

Predicted reactivity
Not reactive in

No confirmed exceptions from predicted reactivity known in the moment.

Additional information
Selected references

To be added when available, antibody released in June 2016.

Application example

western blot using anti-HTA9 antibodies

1) mutant hta9, hta11 about 1 ug protein; 2) mutant hta9, hta11 about 2 ug protein; 3) wild-type about 2-3 ug protein; 4) wild-type about 4-5 ug protein from Arabidopsis thaliana
Protein from Arabidopsis histone preparation (acid extracted, precipitated with acetone and resuspended in urea). Denatured with SDS-PAGE buffer at 90C for 2 min, separated on 15% SDS-PAGE and blotted 1h to PVDF tank transfer. Blots were blocked with PBS + 3% BSA overnight at 4C with agitation. Blot was incubated in the primary antibody at a dilution of 1:1 000 in PBS + 3% BSA for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed once with PBS-T for 5 min. Blot was incubated in secondary antibody PBS + 3% BSA (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602) diluted to 1:25 000 for 1h at RT with agitation. The blot was washed 3-4 times with PBS-T for 5 min. The blot was developed with Immobilion (Millipore) and exposed on an X-ray film for 1 min.

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