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IFR | isoflavone reductase

273 €
AS16 3847 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Oryza sativa

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Item No:
AS16 3847

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product information
Background IFR (isoflavone reductase) is an enzyme involved in isoflavonoid biosynthesis in plants.
Immunogen

KLH-conjugated peptide derived from Oryza sativa UniProt: Q9FTN5, LOC_Os01g01660.1

Host Rabbit
Clonality Polyclonal
Clone
Purity Serum
Format Lyophilized
Quantity 50 ĩl
Reconstitution For reconstitution add 100 ĩl of sterile water.
Storage The antibody may be stored at -20°C for one year in its original formulation. Additionally, antibody may be stored at 2°C to 8°C for up to 1 month without detectable loss of activity. Avoid repeated freeze-thaw cycles of the diluted antibody. Prepared aliquotes.
Tested applications Western blot (WB)
Related products antibodies to proteins involved in response to pathogen attack
Additional information
application information
Recommended dilution 1 : 500 (WB)
Expected | apparent MW 33 | 40 kDa
Confirmed reactivity Oryza sativa
Predicted reactivity Oryza sativa
Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Additional information
Selected references To be added when available, antibody released in October 2016.

Application example 

western blot using anti-IFR, isoflavone reductase antibodies
50-100 µg of total protein from Oryza sativa cv. 9311 flag leaf at maturing stage, extracted with protein extraction reagent were separated on 15% SDS-PAGE and blotted 1h to PVDF using wet transfer. Blots were blocked with 1×TBST+5% Nonfat dry milk for 1h at 37°C with agitation. Blot was incubated in the primary antibody at a dilution of 1: 500 in 1×TBST+5% Nonfat dry milk for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:15 000 in 1x TBST + 5% Nonfat dry milk for 2h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 10-20 seconds.

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