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IBA | Indole-3-butyric acid (rabbit)

339 €
AS13 2744 | clonality: polyclonal | host: rabbit | reactivity: Indole-3-butyric acid

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AS13 2744

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product information
Background

Indole-3-butyric acid (IBA) is a plant hormone belonging to the group of auxins. It is used to stimulate root formation in plant cuttings.

Immunogen BSA-conjugated Indole-3-butyric acid, Target: Indole-3-butyric acid (IBA), CAS no.: 133-32-4(SIGMA), Solubility target: Methanol, ethanol.
Host Rabbit
Clonality Polyclonal
Clone
Purity Protein A purified total IgG
Format Liquid
Quantity 1 ml (2.681 mg/ml)
Reconstitution
Storage 4°C up to one month or in aliquots at -20°C for long time storage. Avoid repeated freezing and thawing.
Tested applications ELISA (ELISA), Immunoaffinity chromatography (IAC)
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other Auxin antibodies

collection of antibodies to plant hormones

Secondary antibodies

Additional information As antibodies to BSA carrier were not removed from this preparation please do not use BSA in your assay and use OVA-conjugated IBA for coating in ELISA.
application information
Recommended dilution 1:50 000 (indirect ELISA), 1:12 800 (direct ELISA)
Expected | apparent MW
Confirmed reactivity Indole-3-butyric acid (IBA)
Predicted reactivity
Not reactive in
Additional information

The antibody may be used in ELISA and Immuno-affinity-chromatography (IAC). Samples may include cutting medium samples and extraction of these samples may be performed using PBS-10% methanol, followed by neutralisation of the extract. 
Antibody titer in ELISA is defined as the dilution that gives 50 % of the absorbance from the maximum absorbance. Plates can be coated with 400 ng/ml ovalbumine-conjugated indole-3-butyric acid. HRP-conjugated anti-rabbit IgG can be used as detecting antibody.

Cross Reactivity              %
Indole-3-aceteic acid    < 0.1 %

Selected references to be added when available, antibody released in November 2013.


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