IRT1 | iron regulated transporter 1

345 €

AS11 1780 | clonality: polyclonal | host: rabbit | reactivity:Arabidopsis thaliana

PRODUCT INFORMATION IN PDF

product information

background

IRT1 is a high afinity iron transported that plays a key role in the uptake of iron (in rhizosphere across the plasma membrane in the root epidermal layer) as well as mediates the heavy metals uptake under iron-defficiency. Is a principal regulator of iron homeaostasis in plants. Synonymes:Fe(2+) transport protein 1, Fe(II) transport protein 1.

immunogen

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana IRT1 sequence, Q38856, At4g19690

antibody format

rabbit

polyclonal

affinity purified serum

lyophilized

quantity

100 µg

for reconstitution add 100 µl, of sterile water.

storage

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. This antibody can be stored in a solution containg 50 % glycerol, final concentration. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

tested applications

western blot (WB)

related products

antibody collection for proteins involved in response to environmental stress

additional information

to be added when available

application information
recommended dilution		1 : 5000 with standard ECL (WB)
expected \| apparent MW		36.7 kDa
confirmed reactivity		Arabidopsis thaliana
predicted reactivity		Noccaea caerulescens, Thlaspi cerulescens
not reactive in		no confirmed exceptions from predicted reactivity are currently known
additional information		to be added when available
selected references		to be added when available, antibody released in March 2012

application example

5 µg of total protein from Arabidopsis thaliana wild type (Col-0) and IRT1 mutant (irt1-1) extracted with SDG buffer (62 mM Tris-HCL pH 8.6, 2.5 % SDS, 2 % dithiothreitol, 10 % glycerol) were separated on 15 % SDS-PAGE and blotted 1h to nitrocellulose. Blots were blocked with 5 % milk in TBST for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 5 000 for 1h at RT with agitation in TBST with 2.5 % milk. The antibody solution was decanted and the blot was rinsed briefly three times, then washed once for 10 min in TBST at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602) diluted to 1:10 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturers instructions. Exposure time was 3 seconds.

Iron-sufficient medium contained 50 µM Fe, +Fe condition, iron-deficient medium 0 µM Fe, -Fe condition.

Courtesy Dr. Petra Bauer and Dr. Rumen Ivanov, Saarland University, Germany

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