JAZ1 | Jasmonate ZIM-domain protein 1

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AS13 2648 | clonality: polyclonal | host: rabbit | reactivity: Arabidopsis thaliana


21 st
Item No:
AS13 2648

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product information

JAZ1 (Jasmonate-ZIM domain containing protein 1)  acts as a repressor of jasmonate responses which are negatively regultated by the proteasome. Interacts with COI1 and inositol pentakisphosphate.


KLH-conjugated synthetic peptide derived from Arabidopsis thaliana JAZ1 protein sequence, UniProt: Q9LMA8, TAIR: AT1G19180

Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum
Format Lyophilized in PBS pH 7.5
Quantity 50 ĩg
Reconstitution For reconstitution add 50 ĩl of sterile water.

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
Related products

AS12 2637 | anti-COI1 | Coronate insensitive 1, rabbit antibodies

Plant protein extraction buffer

Secondary antibodies

Additional information

to be added when available

application information
Recommended dilution

1: 1000 - 1 : 3000 with enhanced ECL (WB)

Expected | apparent MW

27.6 kDa

Confirmed reactivity

Arabidopsis thaliana

Predicted reactivity please inquire
Not reactive in

Oryza sativa

Additional information

to be added when available

Selected references

to be added when available, antibody released in November 2014

application example

western blot using anti-JAZ1 antibodies

JAZ1HA was transiently expressed in Arabidopsis thaliana protoplast for 6 hours. 200 µl of protoplast cells were harvested and mixed with 100 µl 2X SDS buffer. Proteins were separated on 4-12% NuPAGE gel and blotted for 1 hr to PVDF using the Invitrogen XCell II blot module. Blot was blocked with 5% non-fat milk in TBST buffer for 1 hr at room temperature with agitation. Blot was incubated in the primary antibody at a dilution of 1: 3000 over night at 4°C with agitation in TBST with 5% non-fat milk. The blot was rinsed briefly twice, then washed 3 times with TBST for a total of 45 min at RT. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera AS09 602) diluted to 1: 15 000 for 1 hr at RT in TBST with 5% non-fat milk. The blot was washed as above and developed for 5 min using the SuperSignal West Pico Chemiluminescent Substrate (Thermo Scientific) and exposed for 2 min.

Courtesy of Dr. Xuecheng Zhang, Department of Molecular Biology Massachusetts General Hospital, USA

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