ANTIBODY SHOP

Services
Name:
Phone:
E-mail:
Message

KC1 | potassium channel KAT3

371 €

AS09 515 | clonality:polyclonal | host: rabbit | reactivity: Arabidopsis thaliana

PRODUCT INFORMATION IN PDF

Qty: 
Availability:
24 st
Delivery:
Shipping:
Item No:
AS09 515

Info: More information Product suggestions Add review
product information
Background

KC1 is a regulatory K+ channel subunit that assembles with different inward-rectifying K+ channels to affect their activities. The protein is expressed in extremely low amounts, e.g. a few houndred per cell. Alternative names: AKT4, AtKC1, potassium channel TKC

Immunogen

KLH-conjugated peptide derived from Arabidopsis thaliana KC1 protein P92960, At4g32650

Host Rabbit
Clonality Polyclonal
Clone
Purity Affinity purified serum
Format Lyophilized
Quantity 200 ĩg
Reconstitution For reconstitution add 100 ĩl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Related products

AS09 516 | anti-AKT1 | potassium channel AKT1

collection of antibodies to membrane transport system

Plant protein extraction buffer

Secondary antibodies

Additional information For detection images please, refer to the publication belowAntibody detects native and recombinant KC1.
application information
Recommended dilution 1 : 50 with 125I (WB)
Expected | apparent MW

75.5 | 75 kDa

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity

Arabidopsis thaliana

Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Additional information

In the work of Honsbein et al. 125I has been used for detection of KC1 since this was the only way to get enough signal after 2-phase partitioning. ECL+ has been used with the protein after expression in Sf9 insect cells (1: 1000 primary antibody dilution) and in yeast with no problem (single band detected), but these are relatively high expression systems. In native plant material ion channels are expressed in ridiculously small quantities (a few hundred proteins per cell).

Selected references Honsbein et al. (2009). A tripartite SNARE-K+ channel complex mediates in channel-dependent K+ nutrition in Arabidopsis. The Plant Cell 21:2859-2877.


||| For other applications, usage on species other than stated above or any other questions, please use the LiveChat option or contact us at support@agrisera.com