KrP | Kelch repeat-containing protein / galactose oxidase (At3g07720)
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1 : 2000
|Expected | apparent MW||
|Not reactive in||
No confirmed exceptions from predicted reactivity known in the moment.
To be added when available.
|Selected references||To be added when available, antibody released in October 2016.|
XX µg of total protein from Arabidopsis thaliana Col-O root or shoot grown on plates with (+) or without iron (-) extracted and denatured with 2X Laemmli buffer (write exact buffer components) at 95°C for 5 min followed by 5 min on ice. Samples were separated on XX% Mini-PROTEAN TGX Stain-Free gel and blotted XX min to nitrocelulose membrane using semi-dry/wet transfer. Blots were blocked with 5% milk powder dissolved in TBST for XX min at room temperature (RT)/ in a cold room with agitation. Blot was incubated in the primary antibody at a dilution of 1: 2000 for XX min at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed XX times for XX min in TBST buffer at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from ) diluted to 1:4000 in for XX min at RT with agitation. The blot was washed as above and developed for XX min with … (EXACT NAME OF REAGENT AND SUPPLIER) using … detection system (name)/X-ray film. Exposure time was XX seconds.
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