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KrP | Kelch repeat-containing protein / galactose oxidase (At3g07720)

265 €
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AS14 2764  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Arabidopsis thaliana

PRODUCT INFORMATION IN PDF

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AS14 2764

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product information
Background
Kelch repeat-containing protein (KrP)/Galactose oxidase is a protein associated with iron acquisition and metal homeostasis which is a marker for iron defficiency sue to the fact that the transcript levels are stably regulated under iron defficiency conditions. It has been insinuated that to be a target of of bHLH39, bHLH100 and bHLH101 transcription factors. The gene localized at locus At3g07720 is a supposed ancestor of NSP (nitrile-specifier proteins), NSP1, NSP2, and NSP5, which unlike its descendants does not show nitrile-specifier activity.

Alternative names: NSL1, NSP-like1, nitrile-specifier protein-like 1
Immunogen Recombiant KrP derived from Arabidopsis thaliana sequence, UniProt: Q9S7W4, TAIR: At3g07720
Host Rabbit
Clonality Polyclonal
Clone
Purity Serum
Format Lyophilized
Quantity 50 µl
Reconstitution

For reconstitution add 50 ĩl of sterile water.

Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Western Blot (WB)
Related products

anti-IRT1 | Iron regulated transporter 1 antibody
anti-HydA | Iron-hydrogenase HydA1/HydA2 antibody
anti-HydA2 | Iron-hydrogenase HydA2 antibody

Plant protein extraction buffer

Secondary antibodies

Additional information
KrP (NSL1) and NSP5 (nitrile-specifier protein 5) share 53% of amino acid sequence, thus the antibody might also recognize NSP5 from different plant species.
application information
Recommended dilution

1 : 2000 (WB)

Expected | apparent MW

35.7 kDa

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity
Not reactive in

No confirmed exceptions from predicted reactivity known in the moment.

Additional information

This antibody will also bind to recombinant KrP (range 10 ng - 1 µg).

Selected references To be added when available, antibody released in October 2016.

Application example
Western blot with anti-KrP antibody

15 µg of total protein from Arabidopsis thaliana Col-0 root or shoot grown on plates with (+) or without iron (-) extracted and denatured with 2X Laemmli buffer (100 mM Tris pH 6.8; 12 % glycerol, 0.01 % bromophenolblue, 2 % β-mercaptoethanol, 4 % SDS) at 95°C for 5 min followed by 5 min on ice. Samples were separated on 4-20 % Mini-PROTEAN TGX Stain-Free gel and blotted 60 min to nitrocelulose membrane using wet transfer. Blots were blocked with 5% milk powder dissolved in TBST for 30 min at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 2000 for 60 min at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed three times for 15 min in TBST buffer at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera) diluted to 1:4000 in for 60 min at RT with agitation. The blot was washed as above and developed for 2 min with Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare Life Sciences) using the FluorChem Q (protein simple) detection system. Exposure time was 3.2 seconds.


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