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KLH | Keyhole limpet hemocyanin (40 ĩg, HRP-conjugated)

406 €

AS99 001-HRP  |  clonality: polyclonal  |  host: rabbit

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AS99 001-HRP

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product information
Background

Keyhole limpet hemocyanin (KLH) is a large cooper-containing protein consisting of subunits with MW of 400 kDa. It is found in the hemolymph of the sea mollusk Megathura crenulata. This extracellular respiratory protein  has many immunostimulatory properties, including the ability to enhance the host’s immune response by interacting with T cells,  monocytes, macrophages, and polymorphonuclear lymphocytes. Since its discovery, KLH has been used primarily as a carrier for vaccines and antigens and as adjuvant treatment in regimens such as antimicrobial therapy.

Immunogen

Purified keyhole limpet hemocyanin (KLH), whole molecule,

Host Rabbit
Clonality Polyclonal
Clone
Purity Affinity purified serum
Format
Quantity 40 ĩg
Reconstitution
Storage

Store at +4°C for 12-18 months. A preservative may be added for long time storage up to 2 years.

Tested applications Western blot (WB), ELISA (ELISA), immunolocalization (IL)
Related products

AS99 001 | KLH | Keyhole limpet hemocyanin
AS99 002
| anti-BSA rabbit antibody

AS02 023 | anti-BSA hen antibody

Additional information

protein present in plant vascular tissue (xylem and vascular cambium) is detected by anti-KLH antibodies (Höglund et al. 2002) which might lead to false results in IL when using anti-peptide antibodies generated to KLH-conjugated peptide. Further information about it can be found here.

application information
Recommended dilution

1: 10 000 (ELISA), 1: 10 000 (WB), 1: 1000 (IL)

Expected | apparent MW

ca. 400 kDa/subunit

Confirmed reactivity Megathura crenulata - most commonly used carrier protein
Predicted reactivity

Megathura crenulata - most commonly used carrier protein

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

Antibody can be used as a negative control to determine if observed signal is generated by anti-KLH or anti-peptide antibodies. Due to its large size KLH protein will be very difficult to separate on SDS-PAGE.

Selected references


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