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LacI | Lactose operon repressor

376 €

AS16 3144  | Clonality: Monoclonal  |  Host: Mouse  |  Reactivity: LacI from E.coli and A. thaliana

PRODUCT INFORMATION IN PDF

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AS16 3144

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product information
Background LacI (Lactose operon repressor) is a DNA-binding protein which represses the expression of genes encoding proteins responsible for lactose metabolism in bacteria. When lactose in absent from the bacterial medium these genes are repressed and when it becomes available it is converted to allolactose which inhibits LacI binding to DNA, thus allowing lactose metabolism.
Immunogen Recombinant LacI-NLS-6xHis (Lactose operon repressor + C-terminal eukaryotic nuclear localization signal: SSVVHPKKKRKV (NLS) + 6xHis-tag) overexpressed in in E. coli
Host Mouse
Clonality Monoclonal
Clone IgG1, clone 15C11
Purity
Format Liquid in 0.1M sodium phosphate, pH 7.4, 0.15M NaCl, 0.05% (w/v) sodium azide
Quantity 100 µg
Reconstitution
Storage

Store at -20°C. Make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.

Tested applications Western Blot (WB)
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Plant and algal protein extraction buffer

Secondary antibodies

Additional information

This antibody recognizes recombinant and native Lacl.

application information
Recommended dilution 5 µg in 10 ml (WB)
Expected | apparent MW

45 kDa

Confirmed reactivity

LacI from E.coli and A. thaliana

Predicted reactivity Actinopolyspora erythraea, Chlamydia trachomatis, Methanosarcina mazei, Methyloglobulus morosus KOM1, Pseudomonas sp. 1-7, Shigella sonnei, Shinella sp.
Not reactive in

No confirmed exceptions from predicted reactivity are currently known

Additional information
Selected references Kim T., et al. (2005) Engineering a root-specific, repressor-operator gene complex. Plant Biotechnol J. 3(6):571-82.


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