LEA6-3 | late embryogenesis abundant protein 6-3

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AS13 2756 | clonality: polyclonal |  host: rabbit |  reactivity: Phaseolus vulgaris recombiant LEA6-3


14 st
Item No:
AS13 2756

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product information

LEA (Late embryogenesis abundant) proteins are very hydrophilic proteins, described over 25 years ago as accumulating during late stages of plant seed development. Found in vegetative plant tissues following exposure to environmental stress. Synonymes: Putative late embryogenesis abundant protein LEA.


Recombinant PvLEA 6 (original name LEA18) (AF240774.1, gi 8895962) from Phaseolus vulgaris, a group 6 LEA protein (Battaglia et al., 2008)

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 ĩl
Reconstitution For reconstitution add 50 ĩl of sterile water.

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
Related products

AS13 2758 | anti-LEA4-5 | Late embryogenesis abundant protein 4-5, rabbit antibody

Plant protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution

1 : 1000 with standard ECL (WB)

Expected | apparent MW

8.7 | 14 kDa

Confirmed reactivity

recombiant PvLEA6-3

Predicted reactivity Arachis hypogaea, Arabidopsis thaliana, Glycine max, Medicago truncatula, Phaseolus vulgaris
Not reactive in

no confirmed exceptions from predicted reactivity are currently known

Additional information

to be added when available

Selected references

to be added when available, antibody released in August 2014.

application example

western blot using anti-LEA6 antibodies
Recombiant PvLEA6-3 was separated on 12% SDS-PAGE and blotted 1h to nitrocellulose membrane using semi-dry transfer for 1 h. Blots were blocked ON at 4ºC in 2% non-fat milk with agitation. Blots were incubated in the primary antibody at a dilution of 1: 1 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602) diluted to 1:25 000 in 2% non-fat milk for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 1 min.
Recombinant AtLEA4-5 was used as a negative control.

Courtesy of Dr. Alejandra A. Covarrubias, UNAM, Mexico

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