LEA6-3 | late embryogenesis abundant protein 6-3
AS13 2756 | clonality: polyclonal | host: rabbit | reactivity: Phaseolus vulgaris recombiant LEA6-3
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|Recommended dilution||1 : 1000 (WB)|
|Expected | apparent MW||
8.7 | 14 kDa
|Confirmed reactivity||Recombiant PvLEA6-3|
|Predicted reactivity||Arabidopsis thaliana, Arachis hypogaea, Glycine max, Medicago truncatula, Phaseolus vulgaris|
|Not reactive in||No confirmed exceptions from predicted reactivity are currently known.|
to be added when available, antibody released in August 2014.
Recombiant PvLEA6-3 was separated on 12% SDS-PAGE and blotted 1h to nitrocellulose membrane using semi-dry transfer for 1 h. Blots were blocked ON at 4ºC in 2% non-fat milk with agitation. Blots were incubated in the primary antibody at a dilution of 1: 1 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602) diluted to 1:25 000 in 2% non-fat milk for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 1 min.
Recombinant AtLEA4-5 was used as a negative control.
Courtesy of Dr. Alejandra A. Covarrubias, UNAM, Mexico
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