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LexA | SOS function regulatory protein

333 €

AS12 2614  | clonality: polyclonal  |  host: rabbit  |  reactivity:Anabaena sp. strain PCC 7120, Synechocystis sp. strain PCC6803

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Item No:
AS12 2614

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product information
Background

LexA (EC 3.4.21.88) is a DNA binding protein, is associated with SOS response of cyanobacteria as well as other regulatory networs. Has been shown to directly regulate genes involved in carbon assimilation or controlled by carbon availability. In Synechocystis PCC 6803 LexA is not localised to the thylakoid membranes but to the inner region of the cytoplasm 

 

Immunogen

LexA from Anabaena sp. strain PCC 7120 with added His-tag, has been overexpressed in E.coli and purified

Host Rabbit
Clonality Polyclonal
Clone
Purity Serum
Format Lyophilized
Quantity 100 ĩl
Reconstitution For reconstitution add 100 ĩl of sterile water.
Storage

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
Related products

collection of antibodies to other cyanobacterial proteins

Algal protein extraction buffer

Secondary antibodies

Additional information

Antibody can be used as a marker of cytoplasm but also, most likely, a marker for DNA localisation.

application information
Recommended dilution

1 : 16 000 with standard ECL (WB)

Expected | apparent MW

26 kDa (for a monomer/subunit)

Confirmed reactivity Synechocystis sp. PCC 6803 (gene: sll1626, protein: Sll1626)
Predicted reactivity cyanobacteria
Not reactive in

no confirmed exceptions from predicted reactivity are currently known

Additional information

to be added when available

Selected references Oliveira and Lindblad (2011). Novel Insights into the Regulation of LexA in the Cyanobacterium Synechocystis sp. Strain PCC 6803. J. of Bacteriology, Aug. 2011: 3804-3814.


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