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product information
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| background |
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The major light-harvesting antenna complex II (LHCII) in photosynthetic eukaryotes is located in the thylakoid membrane of the chloroplast. It is a heterotrimeric complex formed by up to 3 different individual subtypes of chlorophyll a/b-binding proteins: Lhcb1, Lhcb2, and Lhcb3. Lhcb1 is the most abundant chlorophyll a/b-binding protein in eukaryotic phototrophs and often is coded by several nuclear genes. |
| immunogen |
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BSA-conjugated synthetic peptide derived from Arabidopsis thaliana At1g29910 (Lhcb1.1), At1g29920 (Lhcb1.2), At1g29930 (Lhcb1.3, most expressed), At2g34430 (Lhcb1.4), and At2g34420 (Lhcb1.5) |
| antibody format |
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rabbit |
polyclonal, |
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affinity purified serum in PBS pH 7.4, |
lyophilized |
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| quantity |
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100 µg |
- for reconstitution add 100 µl of sterile water |
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| storage |
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store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. |
| tested applications |
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western blot (WB) |
| related products |
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AS01 002 Lhcb3 | LHCII type III chlorophyll a/b-binding protein AS01 003 Lhcb2 | LHCII type II chlorophyll a/b-binding protein LHC available antibodies against pigment-binding proteins |
| additional information |
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A molecular characterisation of the LHCII proteins can be found in Caffarri et al. (2004) A Look within LHCII: Differential Analysis of the Lhcb1−3 Complexes Building the Major Trimeric Antenna Complex of Higher-Plant Photosynthesis. Biochemistry 43 (29): 9467–9476 |
application information
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| recommended dilution |
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1 : 2500 - 1: 5000 with standard ECL (WB) |
| expected | apparent MW |
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25 | 25 kDa for Arabidopsis thaliana |
| confirmed reactivity |
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Arabidopsis thaliana |
| predicted reactivity |
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angiosperms (monocots and dicots), gymnosperms, mosses, green algae |
| not reactive in |
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no confirmed exceptions from predicted reactivity known in the moment |
| additional information |
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This Lhcb1 antibody is directed specifically against the Arabidopsis Lhcb1 gene products, for those that would prefer higher specific activity over broader specificity offered by Agrisera older Lhcb1 antibody, AS01 004 |
| selected references |
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to be added when available, antibody released in April 2010 |
||| For applications or usage on species others than stated as confirmed above Agrisera offers a payment-after-testing option. To learn more about this or for any questions on this product, please use the LiveChat option in the left menue bar or contact us at support@agrisera.com
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