Plant/Algal cell antibodies / Photosynthesis / LHC

product information

The major light-harvesting antenna complex II (LHCII) in photosynthetic eukaryotes is located in the thylakoid membrane of the chloroplast. It is a heterotrimeric complex formed by up to 3 different individual subtypes of chlorophyll a/b-binding proteins: Lhcb1, Lhcb2, and Lhcb3. Lhcb1 is the most abundant chlorophyll a/b-binding protein in eukaryotic phototrophs and often is coded by several nuclear genes.

BSA-conjugated synthetic peptide derived from a highly conserved sequence of Lhb1 proteins from angiosperms (monocots and dicots) and gymnosperms, including Arabidopsis thaliana At1g29910 (Lhcb1.1), At1g29920 (Lhcb1.2), At1g29930 (Lhcb1.3, most expressed), At2g34430  (Lhcb1.4), and At2g34420 (Lhcb1.5)

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

western blot (WB); immunohistochemistry (IHC), immunogold (IG)

AS01 004-10 Lhcb1 antibody, smaller pack size (10 µg) of AS01 004

AS01 002  Lhcb3 | LHCII type III chlorophyll a/b-binding protein

AS01 003  Lhcb2 | LHCII type II chlorophyll a/b-binding protein

LHC  available antibodies against pigment-binding proteins

recommended secondary antibody

A molecular characterisation of the LHCII proteins can be found in Caffarri et al. (2004) A Look within LHCII:  Differential Analysis of the Lhcb1−3 Complexes Building the Major Trimeric Antenna Complex of Higher-Plant Photosynthesis. Biochemistry 43 (29): 9467–9476

application information

1 : 2000, detected with standard ECL (WB), 1: 100 - 1: 500 (IG)

25 | 25 kDa for Arabidopsis thaliana

Arabidopsis thaliana, Arachis hypogaea, Hordeum vulgare, Lycopersicon esculentum (Solanum lycopersicon), Mesembryanthemum crystallinum, Nicotiana tabacum, Oryza sativa, Pisum sativum, Phaseolus vulgaris, Silene vulgaris, Spinacia oleracea, Zea mays and Chlamydomonas reinhardtii

angiosperms (monocots and dicots), gymnosperms, mosses, green algae

no confirmed exceptions from predicted reactivity known in the moment

a picture from a WB application of this antibody can be found in publications below.

Rudowska et al. (2012). Chloroplast biogenesis - correlation between structure and function. BBA, available on line, March 2012.

Peter et al. (2010). Mg protoporphyrin monomethylester cyclase deficiency and effects on tetrapyrrole metabolism in different light conditions. Plant Cell Physiol. 51(7):1229-1241.

Fristedt et al. (2009). Phosphorylation of photosystem II controls functional macroscopic folding of photosynthetic membranes in Arabidopsis. Plant Cell 21:3950-3964.