Plant/Algal cell antibodies / Photosynthesis / LHC

product information

Lhcbm5 is one of minor LHCII that associates with PSI in state-2

SDS-PAGE purified polypeptide from Chlamydomonas reinhardtii LHCII-type II-enriched fractions

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Western blot (WB)

AS06 117 | anti-Lhcb4 | CP29 (Lhcb4) rabbit antibody

AS09 407 | anti-Lhcb5 CP26 rabbit antibody

Collection of antibodies to Chlamydomonas proteins

This antibody cross-reacts with three major LHCII proteins of Chlamydomonas, which are slightly smaller than Lhcam5 on SDS-gel. 6M urea SDS-PAGE is one of the best systems that separate Lhcbm5 and the other major LHCII proteins. The dilution of the antibody should be carefully determined to reduce the cross-reactions with other major LHCII proteins, we recommend for this purpose to use the dilution of 1: 10 000- 1: 50 000

application information

1: 5000 with standard ECL (WB)

29 | 30 kDa

Chlamydomonas reinhardtii

Chlamydomonas reinhardtii

n.a.

for western blot detection image please refer to the article below

Takahashi et al. (2006). Identification of the mobile light-harvesting complex II polypeptides for state transitions in Chlamydomonas reinhardtii. PNAS 103:477-482.