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product information
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| background |
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LRIG1 protein Q96JA1 is coded by a gene on a chromosome band 3p14.3, this region is known to be deleted in various human cancers. LRIG1 is considered to be a a tumour suppressor gene in humans. LRIG1 is an integral cell-surface membrane protein that is expressed by specific cells in various human tissues and that its 143-kDa form might be cleaved into 111-kDa and 32-kDa fragments. The LRIG1 protein may inhibit the growth of tumors of glial cells and the down-regulation of the LRIG1 gene may be involved in the development and progression of the tumor. |
| immunogen |
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KLH-conjugated synthetic peptide; the peptide corresponds to part of the cytoplasmic tail (C-terminal part) of the protein |
| antibody format |
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rabbit; |
polyclonal; |
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affinity purified IgG in PBS pH 7.4, BSA 1mg/ml; |
lyophilized |
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| quantity |
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100 µg |
- for reconstitution add 90,9 µl of sterile water. |
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| storage |
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store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. |
| tested applications |
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Western blot (WB), immunohistochemistry (IHC) |
| related products |
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AS06 156 | anti-LRIG1 hen antibody |
| additional information |
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to be added when available |
application information
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| recommended dilution |
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1 µg/ml (WB), use at an assay dependent dilution (IHC) |
| expected | apparent MW |
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143 kDa |
| confirmed reactivity |
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human |
| predicted reactivity |
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human |
| not reactive in |
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mouse |
| additional information |
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immunoblotting demonstrated LRIG1 protein in tissue lysates from normal human prostate, mammary epithelial cells, ileum, stomach, lung, and cerebral cortex |
| selected references |
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Nilsson et al. (2003) LRIG1 protein in human cells and tissues. Cell and Tissue Research, 312(1): 65-71. |
application information
western blot  The COS-7 cells transfected with expression vectors encoding LRIG1 (1) or LRIG2 (2) and ZR-75, a breast carcinoma cell line (3). 0.5 microgram of COS-7 lysates and 10 microgram of ZR-75 LRIG1 protein is migrating in denaturing polyacrylamide gel electrophoresis under reducing conditions as two species with apparent molecular weights of 143kDa and 134 kDa, and as two fragments corresponding to an N-terminal 111-kDa species and a C terminal 32-kDa species. Under non-reducing conditions both apparent monomers and apparent higher molecular weight complexes were evident. Immunohistochemistry 0.5 - 1 microgram/ml, done on normal human tissues, showed staining for LRIG1 in epithelia in various organs, scattered neurons, and muscles. Paraffin embedded tissues, detailed protocol can be found in Guo et al. (2006) Acta Neuropathologica 111:238 and Nilsson et al. (2003) Cell and Tissue Research 312:65.
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