LSD1 | Lesion simulating disease 1

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AS13 2746 | clonality: polyclonal | host: rabbit | reactivity: Arabidopsis thaliana



21 st
Item No:
AS13 2746

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product information
Background LSD1 (Lesion simulating disease 1) is a negative regulator of reactive oxygen-induced cell death, cold stress-induced cell death, pathogen-induced hypersensitive response (HR), basal disease resistance. The protein is required for leaf acclimation in response to excess excitation energy. Alternative names: CHILLING SENSITIVE 4, CHS4.

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana LSD1 sequence, UniProt:P94077, TAIR: AT4G20380

Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum
Format Lyophilized in PBS pH 7.4
Quantity 50 ĩg
Reconstitution For reconstitution add 50 ĩl of sterile water.

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
Related products

AS12 1854 | Anti-NPR1 Nonexpresser of PR genes 1, rabbit antibodies
AS12 1867
| anti-HY5 | Protein long hypocotyl 5, rabbit antibodies
AS12 2104 | anti-LSD1 | Lesion simulating disease 1, chicken antibodies available for free for testing

collection of antibodies to developmental biology

Plant protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution

1 : 1000 with standard ECL (WB)

Expected | apparent MW

20 kD

Confirmed reactivity

Arabidopsis thaliana

Predicted reactivity Brassica olereacea, Pisum sativum
Not reactive in

no confirmed exceptions from predicted reactivity are currently known

Additional information
Selected references

Chai et al. (2015). LSD1 and HY5 antagonistically regulate red light induced-programmed cell death in Arabidopsis. Front Plant Sci. 2015 May 5;6:292. doi: 10.3389/fpls.2015.00292. eCollection 2015.

application example

western blot using anti-LSD1 rabbit antibodies

40 µg of total protein from 0.4g leaves extracted with1ml extraction buffer (50 mM Tris-HCl, pH 6.8, 50mM DTT, 4%SDS, 10%glycerol,PVPP) were separated on12 % SDS-PAGE using tank transfer and blotted 1h to PVDF. Blots were blocked with 5% skimmed milk powder for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 4 h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from ) diluted to 1:0 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was second.

Courtesy of Dr. Tingting Chai, South China Normal University, China

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