LSD1 | Lesion simulating disease 1

195 €

AS12 2104 | clonality: polyclonal | host: chicken | reactivity: Arabidopsis thaliana


30 st
Item No:
AS12 2104

Info: More information Product suggestions Add review
product information
Background LSD1 (Lesion simulating disease 1) monitors a superoxide-dependent signal and negatively regulates a plant cell death pathway. contains zinc-finger motifs. LSD1 negatively regulates a basal defense pathway that can act upstream or independently of both NIM1/NPR1 function and SA accumulation. Alternative names: CHILLING SENSITIVE 4, CHS4.

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana LSD1 sequence, UniProt: P94077, TAIR: AT4G20380.

Host Chicken
Clonality Polyclonal
Purity Affinity purfied IgY in PBS pH 7.4 with 0.02 % sodium azide
Format Liquid
Quantity 50 µg

Store at 4°C; make aliquots to avoid working with a stock. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
Related products

AS12 1854 | Anti-NPR1  Nonexpresser of PR genes 1, rabbit antibodies
AS12 1867 | Anti-HY5 | Protein long hypocotyl 5, rabbit antibodies
AS13 2746 | Anti-LSD1 | Lesion simulating disease 1, rabbit antibodies

collection of antibodies to developmental biology

Plant protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution

1 : 1000 with ECL (WB)

Expected | apparent MW

19.5 kD

Confirmed reactivity

Arabidopsis thaliana

Predicted reactivity Brassica olereacea, Pisum sativum
Not reactive in

no confirmed exceptions from predicted reactivity are currently known

Additional information This antibody has so far confirmed reactivity on recombinant LSD1. 
Selected references

To be added when available, antibody released in October 2016. 

Application example

western blot using anti-LSD1 antibodies

50 g of total protein from Arabidopsis thaliana wilde type, lsd1 mutant and transgenic plants overexpressin LSD1-GFP protein under 35S promotor were extracted with following extraction buffer: 0.0625 M Tris-HCl pH 6.8, 1 % SDS, 10 % glycerol, 0.01% beta-mercaptoethanol) were separated in 10 % SDS-PAGE and blotted for 16 hours to PVDF at 4°C using tank transfer. Blots were blocked in PBS-T with 4.5 % non-fat milk for 4 hours at room temperature with agitation. Blot was incubated in the primary antibody at a dilution of 1. 500 for 20 hours at 4°C with agitation. The antibody solution was decanted and the blot was washed 5x for 10 minutes in PBS-T at RT with agitation. Blot was incubated in secondary antibody, anti-chicken IgY, HRP conjugated diluted to 1: 7500 in PBS for 1 hour at RT with agitation. The blot was washed as above and developed for 5 minutes with ECL according to the manufacture's instruction. Exposure time was 1 minute.

Courtesy of Dr. Keun Pyo Lee, SIBS, China

||| For other applications or usage on species other than stated above Agrisera offers a payment-after-testing option. To learn more about this or for any questions please use the LiveChat option or contact us at