MDH | Malate dehydrogenase (cytoplasmic)

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AS13 2706 | clonality: polyclonal | host: rabbit | reactivity:


3 st
Item No:
AS13 2706

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product information

Malate dehydrogenase (EC=  is an enzyme that reversibly catalyzes the oxidation of malate to oxaloacetate. This reaction is part of many metabolic pathways, including the citric acid cycle. Malate dehydrogenase is also involved in gluconeogenesis, the synthesis of glucose from smaller molecules. The protein is highly expressed in: young panicles and immature seeds, while its levels in roots and leaves are low. Alternative name: PP37.


KLH-conjugated synthetic peptide, derived from Oryza sativa cytoplasmic malate dehydrogenase, UniProt: Q7XDC8

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 ĩl
Reconstitution For reconstitution add 50 ĩl of sterile water
Storage The antibody may be stored at -20℃for one year in its original formulation. Additionally, antibody may be stored at 2℃ to 8℃ for up to 1 month without detectable loss of activity. Avoid repeated freeze-thaw cycles of the diluted antibody.
Tested applications Western Blot (WB)
Related products antibodies to proteins involved in carbohydrate metabolism
Additional information
application information
Recommended dilution

1:1000 (WB)

Expected | apparent MW

35 | 35 kDa

Confirmed reactivity

Predicted reactivity Arabidopsis thaliana, Glycine max, Triticum aestivum, Zea mays
Not reactive in
Additional information
Selected references

to be added when available, antibody released in October 2013.

application example

Total protein from Oryza sativa rice (CV. 9311) flag leaf at the flowering stage was ground into a fine powder in liquid nitrogen. An 800 ul aliquot of extraction buffer [62.5 mM TRIS-HCl (pH 7.4), 10% glycerol, 0.1% SDS, 2 mM EDTA, 1 mM phenylmethylsulphonyl fluoride (PMSF), 5% (v/v) b-mercaptoethanol] was added to each 300 mg powder sample. The mixture was vortexed and then chilled on ice for 10 min. Samples were centrifuged at 12,000 rpm for 10min at 4 ℃, and the supernatant was collected and stored at –70 ℃. The protein concentrations of the rice samples were determined using the Bradford method (Bradford, 1976). 20 µg of protein was separated on 12 % SDS-PAGE and blotted 1h to PVDF.
Blots were blocked with for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer’s instructions. Exposure time was 5 min.

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