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MME4 | Malic enzyme

333 €

AS12 2617  | clonality: polyclonal  |  host: rabbit  |  reactivity: Chlamydomonas reinhardtii

 

PRODUCT INFORMATION IN PDF

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AS12 2617

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product information
Background

MME4 (malic enzyme) belongs to the malic enzyme family.

Immunogen

KLH-conjugated synthetic peptide derived Chlamydomonas reinhardtii MME4 protein seqeunce A8IUZ4. The peptide is also conserved in MME3 A8IUW2

Host Rabbit
Clonality Polyclonal
Clone
Purity Affinity purified serum
Format Lyophilized in PBS pH 7.4
Quantity 200 ĩg
Reconstitution For reconstitution add 200 ĩl of sterile water.
Storage

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
Related products

AS12 2618 | anti-FMR | fumarate reductase

Algal protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution

1 : 1000 with standard ECL (WB)

Expected | apparent MW

56 kDa

Confirmed reactivity

Chlamydomonas reinhardtii

Predicted reactivity Chlamydomonas reinhardtii
Not reactive in

Chromera velia

Additional information

to be added when available

Selected references

Subramanian et al. (2014). Profiling Chlamydomonas Metabolism under Dark, Anoxic H 2 ‐Producing Conditions Using a Combined Proteomic, Transcriptomic, and Metabolomic Approach. J Proteome Res. 2014 Oct 21.


application example

western blot using anti-MME4 antibody

25 µg of total protein from Chlamydomonas reinhardtii, oxic conditions and dark anoxia were separated on 4-15 % SDS-PAGE and blotted 1h to PVDF. Blotting was done using SNAP-ID kit: incubation in blocking buffer for 1 min., following incubation in a primary antibody at a dilution of 1: 1 000 for 20 min, wash three times with wash buffer TBS-T, followed by incubation in a secondary antibody at a dilution of 1: 5000, for 20 min. and three times wash in TBS-T.  The blot was washed and developed with AP color development reagent (AP conjugate substrate kit from Bio-rad) according to the manufacturer's instructions. 

Courtesy of Dr. Alexandra Dubini




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