NAB1 | nucleic acid binding protein 1, Chlamydomonas

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AS08 333 | clonality: polyclonal | host: rabbit | reactivity: Chlamydomonas reinhardtii | cellular [compartment marker] of Chlamydomonas cytoplasm


27 st
Item No:
AS08 333

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product information

In Chlamydomonas reinhardtii NAB1 (nucleic acid binding protein 1) interacts in the cytosol with mRNAs coding for light-harvesting proteins of Photosystem II. NAB1 has structural similarities (cold-shock domain, CSD) to the FRGY2-protein from Xenopus laevis. According to immunolocalization studies NAB1 protein was exclusively found in cytosol, Mussgnug et al. (2005).


His-tagged recombinant NAB1 from Chlamydomonas reinhardtii (Q8GV23)

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 ĩl
Reconstitution For reconstitution add 50 ĩl of sterile water.

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB), immunoprecipitation (IP)
Related products

Collection of antibodies to Chlamydomonas proteins

Additional information

NAB1 can be used as a marker of cytoplasm in Chlamydomonas reinhardii.

application information
Recommended dilution

1: 10 000 standard ECL (WB)

Expected | apparent MW

26.5 kDa | 26 kDa (for Chlamydomonas reinhardtii)

Confirmed reactivity

Chlamydomonas reinhardtii

Predicted reactivity

Chlamydomonas reinhardtii

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

to be added when available

Selected references Craft et al. (2015). Tubulin transport by IFT is upregulated during ciliary growth by a cilium-autonomous mechanism. J Cell Biol. 2015 Jan 19;208(2):223-37. doi: 10.1083/jcb.201409036. Epub 2015 Jan 12.
Desai et al. (2014). Chlamydomonas axonemal dynein assembly locus ODA8 encodes a conserved flagellar protein needed for cytoplasmic maturation of outer dynein arm complexes. Cytoskeleton (Hoboken). 2014 Dec 31. doi: 10.1002/cm.21206.
Mussgnug et al. (2005) NAB1 is an RNA binding protein involved in the light-regulated differential expression of the light-harvesting antenna of Chlamydomonas reinhardtii. Plant Cell 17:3409-3421

application example

Western blot obtained using 1:10000 anti-NAB1 (AS08 333) and 1:160000 AP-conjugated secondary antibody on (1) recombinant NAB1 protein, (2) cytosolic protein of Chlamydomonas reinhardtii wild strain Cw10, (3) NAB1-deficient strain Stm3, and (4) Stm3 complemented with NAB1.

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