NBR1 | Autophagy substrate NBR1

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AS14 2805 | clonality: polyclonal | host: rabbit | reactivity:Arabidopsis thaliana, Physcomitrella patens


3 st
Item No:
AS14 2805

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product information
Background NBR1 (Autophagy substrate NBR1) is involved in selective autophagy process of damaged organelles, intracellular microbes, protein aggregates, cellular structures and specific soluble proteins. NBR1 mediates the process as an autophagic adapter. The protein has two UBA domains but only the C-terminal UBA domain bound ubiquitin. Alternative names: At4g24690, Putative uncharacterized protein F22K18.110.
Immunogen UNA2 domain of NBR1 of Arabidopsis thaliana, fused with GST, UniProt:Q9SB64, TAIR:AT4G24690
Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 ĩl
Reconstitution For reconstitution add 50 ĩl of sterile water.

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
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Plant and algal protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution

1 : 500 - 1: 5000 with standard ECL (WB)

Expected | apparent MW

75 | 100 kDa

Confirmed reactivity

Arabidopsis thaliana, Physcomitrella patens

Predicted reactivity Brassicaceae family
Not reactive in

no confirmed exceptions from predicted reactivity are currently known

Additional information

specific extraction method and tissue type needs to be used as described in Minina et al. (2013). Dilution in western blot depends upon amount of NBR1 in the sample.

Selected references Hackenberg et al. (2013). Catalase and NO CATALASE ACTIVITY1 promote autophagy-dependent cell death in Arabidopsis. Plant Cell. 2013 Nov;25(11):4616-26. doi: 10.1105/tpc.113.117192. Epub 2013 Nov 27. 
Munch et al. (2013). Autophagy deficiency leads to accumulation of ubiquitinated proteins, ER stress, and cell death in Arabidopsis. Autophagy. Autophagy. 2014 Sep 1;10(9):1579-87. doi: 10.4161/auto.29406. Epub 2014 Jul 7.
Minina et al. (2013). Autophagy mediates caloric restriction-induced lifespan extension in Arabidopsis. Aging Cell. 2013 Apr;12(2):327-9. doi: 10.1111/acel.12048. Epub 2013 Feb 28. (method description in supplemental materials)
Katsiarimpa et al. (2013). The Deubiquitinating Enzyme AMSH1 and the ESCRT-III Subunit VPS2.1 Are Required for Autophagic Degradation in Arabidopsis.
Svenning et al. (2011). Plant NBR1 is a selective autophagy substrate and a functional hybrid of the mammalian autophagic adapters NBR1 and p62/SQSTM1. Autophagy. 2011 Sep;7(9):993-1010. Epub 2011 Sep 1. (original reference)

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