Animal cell antibodies / Neurosteroids/Neurobiology

product information

Nitrotyrosine is a marker of NO-dependent oxidative stress. It is a product of tyrosine nitration mediated by reactive nitrogen species.

BSA-conjugated 3-(4-Hydroxy-3-nitrophenyl
acetamido) propionic acid

store at -20°C; avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

western blot (WB), ELISA (ELISA), immunohistochemistry (IHC), immunoprecipitation (IP)

AS10 706-100 | anti-nitrotyrosine mouse antibody, 100 µg

1 mg/ml of Protein G purified IgG2A in PBS pH 7.4,  0.09 % sodium azide, 50 % glycerol

application information

needs to be determined for each application

n.a.

human, mouse, rat, dog

higher plants

no confirmed exceptions from predicted reactivity known in the moment

antibody recognizes 3-nitrotyrosine moieties. No detectable crossreactivity
with non-nitrated tyrosine. Not species specific.

0.7μg/ml was sufficient for detection of 5 μg SIN-1 treated BSA by Western Blot using standard ECL

Antibody used in western blot and ELISA:  Girault et al. (2001).Immunodetection of 3-nitrotyrosine in the liver of zymosan-treated rats with a new monoclonal antibody: comparison to analysis by HPLC. Free Radical Biology and Medicine, 31 (11): 1375-1387. Gow et al. (2004).Biological significance of nitric oxide-mediated protein modifications. Am J Physiol Lung Cell Mol Physiol. 287(2): L262-8.

Antibody used in immunohistochemistry: Pfister et al. (2002). Inducible nitric oxide synthase and nitrotyrosine in listeric encephalitis: a cross-species study in ruminants. Vet Pathol. 39: 190-199.