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NodGS | Nodulin / glutamate-ammonia ligase-like protein

298 €
Buy 2 items of this product for 213 €/each
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AS15 3030A | clonality: polyclonal | host: rabbit | reactivity: Arabidopsis thaliana

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AS15 3030A

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product information
Background

NodGS (Nodulin/glutamate-ammonia ligase-like protein) shares homology with nodulins and self-assembles into oligomers which are present in response to flagellin treatment.  It contains a C-terminal domain of a prokaryotic glutamine synthetase type I. Protein is mainly localized to cytoplasm and is present in a oligomeric form of ca. 700 kDa.

Immunogen

KLH-conjugated synthetic peptide derived from a C-terminal of Arabidopsis thaliana NodGS, UniProt: Q8W473 , TAIR: AT3G53180

Host Rabbit
Clonality Polyclonal
Clone
Purity Affinity purified serum
Format Liquid with glycerol at 50 %
Quantity 50 ĩg
Reconstitution For reconstitution add 50 ĩl of sterile water.
Storage

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB), immunoprecipitation (IP), immunofluorescence (IF)
Related products

AS05 068 | anti-GDH1 | glutamate dehydrogenase 1, rabbit antibody

AS08 296 GLN | anti-GS2, chloroplastic form of glutamine synthetase, rabbit antibody
AS08 295PRE | GLN1 GLN2 | GS1 glutamine synthetase, pre-immune serum

collection of antibodies to proteins involved in nitrogen metabolism

plant and algal protein extraction buffer

secondary antibodies

Additional information
application information
Recommended dilution 1:2000 (WB), 1:300-1:400 (IP), 1:3000 (IF)
Expected | apparent MW

94 | 90 kDa

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Medicago truncatula, Theobroma cacao
Not reactive in

no confirmed exceptions from predicted reactivity known presently

Additional information

to be added when available

Selected references Doskocilova et al. (2011). A nodulin/glutamine synthetase-like fusion protein is implicated in the regulation of root morphogenesis and in signalling triggered by flagellin. Planta 234, 459–476. doi: 10.1007/s00425-011-1419-7


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