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NtrC | Thioredoxin reductase (TR/TRxR)

371 €

AS06 159 |  clonality: polyclonal | host: rabbit  | reactivity: Chlamydomonas reinhardtii

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AS06 159

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product information
Background

Thioredoxin Reductase (TR, TrxR) is the only known enzyme (EC 1.8.1.9) which is reducing thoredoxin (Trx). Activity of thoredoxin is essential for growth and survival of the cell. There seem to be one isoform of NtrC protein in Arabidopsis which includes an N-terminal reductase domain and a C-terminal domain related to thioredoxin proteins. Arabidopsis thaliana and Chlmydomonas reinhardtii NtrC proteins are ca. 568 amino acids long, but include ca. 80 amino acid signal peptides, for mature protein   size of ca. 488 amino acids.

Immunogen

KLH-conjugated peptide derived from N-terminal domain of NtrC of Chlamydomonas reinhardtii A8HNQ7

Host Rabbit
Clonality Polyclonal
Clone
Purity Affinity purified serum
Format Lyophilized in PBS pH 7.4
Quantity 200 ĩg
Reconstitution For reconstitution add 100 ĩl of sterile water.
Storage

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
Related products

Collection of antibodies to Chlamydomonas proteins

Algal protein extraction buffer

Secondary antibodies

Additional information

Peptide target chosen from the N-terminal domain, nearly fully conserved within 3-4 NtrA-related proteins from Arabidopsis thalina and with NtrC related proteins from Chlamydomonas reinhardtii and Synechococcus sp. 7942 and other cyanobacteria

application information
Recommended dilution

1 : 500 with standard ECL (WB)

Expected | apparent MW

55.3 kDa

Confirmed reactivity

Chlamydomonas reinhardtii

Predicted reactivity

Chlamydia sp. Ostreococcus luminarius, Physcomitrella patens, Synechococcus sp.

Not reactive in

Arabidopsis thaliana

Additional information

antibody will react with long NtrC of Chlamydomonas reinhardtii proteins and shorter NtrA-type rpoteins, which would be distinguished by migration size.

Selected references


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