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p15 [Peanut clump virus]

265 €
Buy 2 items of this product for 198 €/each
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AS16 3983 |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: p15 [Peanut clump virus]

PRODUCT INFORMATION IN PDF

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AS16 3983

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product information
Background
Immunogen Recombinant p15 [Peanut clump virus] Protein accession number: NP_620041.
Host Rabbit
Clonality Polyclonal
Clone
Purity Serum
Format Lyophilized
Quantity 50 µl
Reconstitution
Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Western Blot (WB)
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Additional information
application information
Recommended dilution

1: 5000 (WB)

Expected | apparent MW

14.6 | 15 kDa

Confirmed reactivity

p15 [Peanut clump virus]

Predicted reactivity
Not reactive in No confirmed exceptions from predicted reactivity known at the moment.
Additional information
Selected references To be added when available, antibody released in September 2016.

application example

western blot using anti-p15 [Peanut clump virus] antibodies

The indicated amounts of the untagged recombinant proteins (extracted in 2 x SDS buffer (0.125M Tris pH 6.8, 4% (w/v) SDS, 20%(v/v) glycerol, 0.2M DTT, 0.02% bromophenol blue)) was separated on 15% SDS-PAGE and blotted 1h to PVDF membrane. Filters were blocked 1h with 5% low-fat milk powder in PBS-T (1 X PBS buffer; 0.5% TWEEN20) and probed with the serum of anti-FNY 2b antibody (1:5 000, 1h) and secondary anti-rabbit (1:5000, 1 h) antibody (HRP conjugated) in PBS-T containing 5% low fat milk powder. Antibody incubations were followed by washings in PBS-T. All steps were performed at RT with agitation. Blots were developed for 5 min with ECL-Prime detection reagent according the manufacturer's instructions (GE Healthcare: RPN2236). Exposure time was 20 seconds.

Courtesy of Dr. Xiuren Zhang, Texas A&M University, USA

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