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PAD4 | Phytoalexin deficient 4

265 €
Buy 2 items of this product for 198 €/each
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AS13 2750 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

PRODUCT INFORMATION IN PDF

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AS13 2750

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product information
Background PAD4 (Phytoalexin deficient 4) is a lipase-like protein that is important for salicylic acid signaling and functions in resistance (R) gene-mediated and basal plant disease resistance. Can interact directly with EDS1, another disease resistance signaling protein. PAD4 participates in the regulation of various molecular and physiological processes.

Alternative names: Lipase-like PAD4 (EC:2.3.1.-), Protein ENHANCED DISEASE SUSCEPTIBILITY 9, Protein PHYTOALEXIN DEFICIENT 4, AtPAD4.
Immunogen

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana PAD4 sequence, UniProt: Q9S745, TAIR: AT3G52430

Host Rabbit
Clonality Polyclonal
Clone
Purity Affinity purified serum in PBS pH 7.4.
Format Lyophilized
Quantity 50 µg
Reconstitution

For reconstitution add 50 µl, of sterile water.

Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications

Western Blot (WB)

Related products AS13 2751 | Anti-EDS1 | Enhanced disease susceptibility 1, rabbit antibodies
AS15 2906 | Anti-MKK4 | Mitogen-activated protein kinase kinase 4, rabbit antibodies
AS10 687 | Anti-PR-1 | Pathogenesis-related protein 1, rabbit antibodies
AS12 2373 | Anti-PR-5 | Pathogenesis-related protein 5 (A.thaliana), rabbit antibodies

Plant protein extraction buffer
Secondary antibodies

Additional information
application information
Recommended dilution

1 : 1000 (WB)

Expected | apparent MW 60.9 kDa
Confirmed reactivity

Arabidopsis thaliana

Predicted reactivity Brassica napus, Capsella rubella, Eutrema salsugineum, Oryza sativa
Not reactive in

no confirmed exceptions from predicted reactivity are currently known

Additional information
Selected references

To be added when available, antibody released in April 2017. 


Application example

Western blot using anti-PAD4 antibodies




5 µg of protein from Arabidopsis thaliana Col0 and pad4-5 mutant leaf tissue, extracted with Agrisera PEB protein extraction buffer 1X (AS08 300) were separated on 12 % SDS-PAGE using mini-protean tank (BioRad system) to transfer to nitrocellulose membrane during 1 hour at 70 mV. Blots were blocked with unfatty milk overnight at 4 ºC with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera AS09 602) diluted to 1:10 000 for 1h at RT with agitation. The blot was washed as above and developed for 5 min with SuperSignal™ West Femto Maximum Sensitivity Substrate according to the manufacturer's instructions. Exposure time was 30 seconds. For transference control the membrane was stained with Ponceau red and integrity of proteins was evaluated using 10% SDS-PAGE coomassie stained.

pad4-5, T-DNA knockout mutant, reference: Feys et al. (2001). Direct interaction between the Arabidopsis disease resistance signaling proteins, EDS1 and PAD4. EMBO J.: 20:5400–5411.

Courtesy of Dr. Rodrigo A. Contreras, Laboratorio de Fisiología y Biotecnología Vegetal, Universidad de Santiago de Chile, Chile

||| For other applications, usage on species other than stated above or any other questions, please use the LiveChat option or contact us at support@agrisera.com