PEPC | Phosphoenolpyruvate carboxylase
AS09 458 | clonality:polyclonal | host:rabbit | reactivity: A. comosus, A. thaliana, S. PCC 6803, Ch. velia, F. bidentis, H. vulgare, J. curcas, Lupinus sp. , M. crystallinum, N. tabacum, O. sativa, Saccharum spp. hybrid clone C91-301, S. laricifolia, Z. mays, Synechocystis sp. , P. tricornutum (strain CCAP 1055/1), T. weissfloggi
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1 : 1000 with standard ECL (WB), 1: 500 (IL)
|Expected | apparent MW||
110 | 105 kDa
Ananas comosus, Arabidopsis thaliana, Synechocystis PCC 6803, Chromera velia, Hordeum vulgare, Jatropha curcas, Lupinus sp. , Mesembryanthemum crystallinum, Nicotiana tabacum, Oryza sativa, Saccharum spp. hybrid clone C91-301, Salsola laricifolia, Synechocystis sp. , Phaeodactylum tricornutum (strain CCAP 1055/1), Thalassiosira weissfloggi, Zea mays
Cucumis sativus (PEPC1, PEPC2, PEPC3), Flaveria bidentis, Flaveria trinervia, Glycine max, Lupinus albus, Mammillaria thornberi, Manihot obovata, Medicago sativa, Morinda citrifolia, Nopalea gaumeri, Opuntia macbridei, Pachycereus pringlei, Solanum tuberosum, Spinacia oleracea, Streptanthus tortuosus, Pachycereus hollianus, Pisum sativa, Phaseolus vulgaris,Triticum aestivum, algae, diatoms: Thalassiosira pseudonana, other species: Salmonella sp., Schiedea hookeri, Shigella sp. Schiedea sarmentosa, Streptanthus farnsworthianus, Tacinga saxatilis,Yersinia sp. Vibrio sp.
|Not reactive in||
No confirmed exceptions from predicted reactivity known in the moment
|Additional information||Antibody can be also used following 2D gel electrophoresis.|
|Selected references||Ribeiro et al. (2017). Increased sink strength offsets the inhibitory effect of sucrose on sugarcane photosynthesis. J Plant Physiol. 2017 Jan;208:61-69. doi: 10.1016/j.jplph.2016.11.005.
Shen et al. (2016). The existence of C4-bundle-sheath-like photosynthesis in the mid-vein of C3 rice. Rice (N Y). 2016 Dec;9(1):20. doi: 10.1186/s12284-016-0094-5. Epub 2016 May 10.
Ishikawa et al. (2016). NDH-Mediated Cyclic Electron Flow Around Photosystem I is Crucial for C4 Photosynthesis. Plant Cell Physiol. 2016 Aug 6. pii: pcw127. [Epub ahead of print]
Shen et al. (2015). Overexpression of maize phosphoenolpyruvate carboxylase improves drought tolerance in rice by stabilization the function and structure of thylakoid membrane. Photosynthetica, September 2015, Volume 53, Issue 3, pp 436-446.
Foley et. al (2015). Analysis of conglutin seed storage proteins across lupin species using transcriptomic, protein and comparative genomic approaches. BMC Plant Biology 2015, 15:106 doi:10.1186/s12870-015-0485-6.
Shi et al. (2015). Phosphoenolpyruvate carboxylase in arabidopsis leaves plays a crucial role in carbon and nitrogen metabolism. Plant Physiol. 2015 Mar;167(3):671-81.
Kataya et al. (2015). Protein Phosphatase 2A Holoenzyme Is Targeted to Peroxisomes by Piggybacking and Positively Affects Peroxisomal β-Oxidation. Plant Physiol. 2015 Feb;167(2):493-506. doi: 10.1104/pp.114.254409.
Wen and Zhang (2014). Salsola laricifolia, another C 3 –C 4 intermediate species in tribe Salsoleae s.l. (Chenopodiaceae). Photosynth Res. 2014 Sep 17. (immunolocalization)
Suk Chang et al. (2014). Improvement of the phosphoenolpyruvate carboxylase activity of Phaeodactylum tricornutum PEPCase 1 through protein engineering. Enzyme and Microbial Technology, April 20134.
Sharwood et al. (2014). Photosynthetic flexibility in maize exposed to salinity and shade. J Exp Bot. 2014 Apr 1.
5 µg of total protein from (1) Arabidopsis thaliana leaf extracted with Protein Extration Buffer, PEB (AS08 300), (2) Spinacia oleracea total cell, extracted with PEB, (3) Hordeum vulgare total cell extracted with PEB, (4) Zea mays total cell extracted with PEB, were separated on 4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to PVDF. Blots were blocked immediately following transfer in 2% ECL Advance blocking reagent (GE Healthcare) in 20 mM Tris, 137 mM sodium chloride pH 7.6 with 0.1% (v/v) Tween-20 (TBS-T) for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 10 000 for 1h at room temperature with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Abcam) diluted to 1:50 000 in 2% ECL Advance blocking solution for 1h at room temperature with agitation. The blots were washed as above and developed for 5 min with ECL Advance detection reagent according the manufacturers instructions. Images of the blots were obtained using a CCD imager (FluorSMax, Bio-Rad) and Quantity One software (Bio-Rad).
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