Cyt b6 | (PetB) thylakoid membrane cytochrome b6 protein

340 €

AS15 2878  | clonality: polyclonal  |  host: rabbit  |  reactivity: Arabidopsis thaliana


18 st
Item No:
AS15 2878

Info: Product suggestions Add review
product information

PETB is a subunit of the cytochrome b6 complex, this protein mediates electron transfer between photosystem II and photosystem I and cyclic electron flow around photosystem I.


Recombinant protein from Arabidopsis thaliana

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 100 ĩl

For reconstitution add 100 µl of sterile water.

Lyophilized antibody can be stored at -20 or -80°C.
Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
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AS03 034
 | anti-Cyt b6 | (PetB) thylakoid membrane cytochrome b6 protein, N terminal, rabbit antibodies 
collection of antibodies to proteins of photosynthetic electron transfer

Secondary antibodies

Additional information
application information
Recommended dilution

1:3000 (WB)

Expected | apparent MW

24.15 kDa

Confirmed reactivity

Arabidopsis thaliana

Predicted reactivity

Arabis hirsuta, Cardamine resedifolia, Iochroma stenanthum, Oenothera sp., Nasturtium officinale, Nicotiana tabacum, Raphanus sativus, Salvia miltiorrhiza

Not reactive in

no confirmed exceptions from predicted reactivity are currently known

Additional information
Selected references to be added when available, antibody released in May 2015

application example

Respective amounts of recombinant Arabidopsis thaliana PetB were separated on 12 % SDS-PAGE  and blotted 1h to PVDF using tank transfer. Blots were blocked with 5 % milk in TBS  for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 3000 decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in  for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 7 seconds.

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