ANTIBODY SHOP

Services
Name:
Phone:
E-mail:
Message

PIF4 | Phytochrome interacting factor 4

265 €
Buy 2 items of this product for 198 €/each
Buy 3 items of this product for 180 €/each

AS16 3955 | Clonality: Polyclonal | Host: Goat | Reactivity: Arabidopsis thaliana

PRODUCT INFORMATION IN PDF

Qty: 
Availability:
28 st
Delivery:
Shipping:
Item No:
AS16 3955

Info: Product suggestions Add review
product information
Background PIF4 (PHYTOCHROME INTERACTING FACTOR 4) is a transcription factor involved in the phytochrome B signaling pathway. Interacts with APRR1/TOC1 and PIF3 and binds to EGL2 and RGA. Alternative names: AtPIF4, Basic helix-loop-helix protein 9, AtbHLH9, bHLH 9, SRL2, Short under red-light 2, EN102, Transcription factor EN 102, bHLH transcription factor bHLH009.
Immunogen KLH-conjugated peptide derived from Arabidopsis thaliana PIF4, UniProt:Q8W2F3, TAIR:AT2G43010
Host Goat
Clonality Polyclonal
Clone
Purity Affinity purified serum
Format
Quantity 50 µg
Reconstitution

For reconstitution add 50 µl of sterile water.

Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles.

Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications

Western Blot (WB)

Related products

AS12 1870 | anti-PIF3 | Phytochrome interacting factor 3, rabbit antibody
AS11 1630 | anti-RGA | DELLA protein RGA, rabbit antibody
AS11 1803
| anti-RGA-like protein 2 | DELLA protein RGL2, rabbit antibody
AS12 2646 | anti-TOC1 | TIMING OF CAB EXPRESSION 1, rabbit antibody

Plant protein extraction buffer

Secondary antibodies

Additional information

PIF proteins are not that stable, therefore special precautions should be taken during extraction and whole procedure should be performed in as little light as possible (light green light).

Extraction of PIF proteins is described in Shen et al. (2007).

application information
Recommended dilution 1: 1000 (WB)
Expected | apparent MW 48.3 | 60 kDa
Confirmed reactivity

Arabidopsis thaliana

Predicted reactivity for other species - please, inquire
Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information
Selected references

to be added when available, antibody released in February 2014.


Application example


western blot using anti-PIF4 goat antibody


30 ul of total protein from Arabidopsis thaliana grown in 12 hour light/ 12 hour dark growth conditions and 6-day-old seedling samples taken at the end of the day (ZT12) were extracted with 100 mM MOPS, pH 7.6, 100 mM NaCl, 10% glycerol, 40 mM 2-mercaptoethanol, 5% SDS, 1X protease inhibitor cocktail from Roche, 2 mM PMSF. 80 µl buffer were added to about 100 µl grinded powder, then immediately heated at 70ºC for 10 minutes and separated on 8 % Bis-Tris SDS-PAGE and blotted 1h to PVDF using semi-dry or tank transfer. Blots were blocked with 5 % non-fat milk in TBST for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for over night at 4ªC with agitation in TBS-T. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5 minutes with Thermo DURA (Thermofisher). Exposure time was 1 minute.

Courtesy of Dr. Bo Zhang, Umeå Plant Science Centre, Sweden

||| For other applications or usage on species other than stated above Agrisera offers a payment-after-testing option. To learn more about this or for any questions please use the LiveChat option or contact us at support@agrisera.com