PIP2;3 | aquaporin, plasma membrane intrinistic protein 2-3

PIP2;1 fuctions as a silicon transporter which helps plants to overcome abiotic and biotic stress. Alternative names: aquaporin NIP2-1,NOD26-like intrinsic protein 2-1 OsNIP2;1, Silicon transporter LSI1, Low silicon protein 1

KLH-conjugated peptide derived from Oryza sativa PIP2;3  Q7XUA6

store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.

ELISA (ELISA), western blot (WB), immunogold (IG)

collection of antibodies to plasma membrane proteins

AS09 505 | anti-PIP1s | aquaporin

AS09 507 | anti-PIP2;1 | aquaporin, plasma membrane intrinistic protein 2-1

AS09 508 | anti-PIP2;5 | aquaporin, plasma membrane intrinistic protein 2-5

0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.

Antibodies will detect target protein in a few  µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient.

For immunogold technique, secondary antibodies conjugated with coloidal gold, 10 nm particle size were used.

Protocol for isolation of plasma membrane can be found here.