Plant/Algal cell antibodies / Hormones / Auxins

product information

Indole 3 acetic acid (IAA) is the principal auxin in higher plants. This hormone is produced in  in cells in the apex and young leaves of a plant. Plant cells synthesize IAA from tryptophan. Different effects caused by auxines include: induction of cell elongation and cell division and have a subsequent results for plant growth and development.

BSA-conjugated, via C1 carobxyl group, indole-3-acetic acid (N1)

store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

immunolocalization (IL)

AS06 193| anti- IAA

AS09 420 | anti- indole-3-acetic acid (C1') for ELISA

useful references for immunolocalization work: DeWitte et al. (1999). Dynamics of cytokinins in apical shoot meristems of a day neutral tobacco during floral transition and flower formation. Plant Physiol. 119:111-121. Shiozaki et al. (1999). Localisation of abscisic acid in grape berry by immunohitochemical techniques. J.Japan Hort.Sci.68:1-9.  Skene et al. (1987). Model systems for the immunolocalisation of cis, trans-abscisic acid in plant tissues. Planta 172:192-199. Sosountozov et al. (1986). Immunoelectron-microscopy localisation of abscisic acid with collodial gold on Lowicryl-embeded tissues of Chenopodium polyspermum L. Planta 168: 471-481. Zavala et al. (1983). Localisation of a phytohormone using immunocytochemistry. J.Cell Biol. 97:1235-1239.

application information

specific information about dilution is going to be included on a vial

n.a.

indole-3-acetic acid (N1)

indole-3-acetic acid (N1)

no confirmed exceptions from predicted reactivity known in the moment

Antibody can be used for direct detection of free IAA (see reference below)

Antibody is provided in 50% glycerol. For larger quantity (1 mg) or for  ELISA kit - please inquire.

Schlicht et al. (2006) Auxin Immunolocalization Implicates Vesicular Neurotransmitter-Like Mode of Polar Auxin Transport in Root Apices. Plant Signalling & Behaviour 1(3):
122-133.