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Plant/Algal cell antibodies / Bioenergetics


Ca2+-ATPase | calmodulin-stimulated calcium-ATPase

Art no: AS09 486
Price: 392
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product information

background  

Ca2+-ATPase | calmodulin-stimulated calcium-ATPase belongs to family of catoin transport ATPase (P-type).

immunogen  

KLH-conjugated synthetic peptide derived from Brassica oleracea calmodulin-stimulated calcium-ATPase P93067

antibody format  

rabbit

polyclonal,

serum,

quantity  

100 µl

storage  

store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.

tested applications  

western blot (WB), ELISA (ELISA)

related products  

collection of antibodies to tonoplast proteins

additional information  

0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.

This protein is of low abundance in plant tissues.

Protocol for tonoplast membrane isolation can be found here.

application information

recommended dilution  

1: 8000 (ELISA), 1: 1000 with standard ECL (WB)

expected | apparent MW  

111 | 110 kDa (Raphanus sativus)

confirmed reactivity  

Arabidopsis thaliana, Brassica oleracea, Raphanus sativus

predicted reactivity  

dicots including: Vitis vinifera, monocots including: Oryza sativa, Triticum aestivum, trees: Populus balsamifera, moss: Physcomitrella patens

not reactive in  

no confirmed exceptions from predicted reactivity known in the moment

additional information  

Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.

Manufactured by Operon Biotechnologies.

selected references  

to be added when available 


application example

 

 

1 µg and 10 µg of crude membrane fraction/lane from Raphabus sativa L. Tokinashi-daikon  were separated on 12 % SDS-PAGE and blotted 1h to PVDF membrane (40 min. at 10 V using BioRad semidry transfer). Filters were blocked 1h with 5 % low-fat milk powder in TBS-T (0.05% Triton X.100). Membranes were washed 5 times with TBS-T, each time in a fresh polystyrene box and probed with anti-Ca2+ ATPase  antibodies (AS09 486, 1:1000, 1h) and secondary anti-rabbit (1:2000, 1 h). All steps were performed in RT with agitation.

 

  western blot using Ca2+ ATPase antibodies

||| For applications or usage on species others than stated above Agrisera offers a payment-after-testing option. To learn more about this or for any questions on this product, please use the LiveChat option in the left menue bar or contact us at  support@agrisera.com



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