store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.

tested applications

western blot (WB), ELISA (ELISA)

related products

collection of antibodies to tonoplast proteins

additional information

0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.

This protein is of low abundance in plant tissues.

Protocol for tonoplast membrane isolation can be found here.

application information
recommended dilution		1: 8000 (ELISA), 1: 1000 with standard ECL (WB)
expected \| apparent MW		111 \| 110 kDa (Raphanus sativus)
confirmed reactivity		Arabidopsis thaliana, Brassica oleracea, Raphanus sativus
predicted reactivity		dicots including: Vitis vinifera, monocots including: Oryza sativa, Triticum aestivum, trees: Populus balsamifera, moss: Physcomitrella patens
not reactive in		no confirmed exceptions from predicted reactivity known in the moment
additional information		Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel. Manufactured by Operon Biotechnologies.
selected references		to be added when available

application example

1 µg and 10 µg of crude membrane fraction/lane from Raphabus sativa L. Tokinashi-daikon were separated on 12 % SDS-PAGE and blotted 1h to PVDF membrane (40 min. at 10 V using BioRad semidry transfer). Filters were blocked 1h with 5 % low-fat milk powder in TBS-T (0.05% Triton X.100). Membranes were washed 5 times with TBS-T, each time in a fresh polystyrene box and probed with anti-Ca2+ ATPase antibodies (AS09 486, 1:1000, 1h) and secondary anti-rabbit (1:2000, 1 h). All steps were performed in RT with agitation.

western blot using Ca2+ ATPase antibodies

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