Plant/Algal cell antibodies / Bioenergetics

product information

Ca2+-ATPase | calmodulin-stimulated calcium-ATPase belongs to family of catoin transport ATPase (P-type).

KLH-conjugated synthetic peptide derived from Brassica oleracea calmodulin-stimulated calcium-ATPase P93067

store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.

western blot (WB), ELISA (ELISA)

collection of antibodies to tonoplast proteins

0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.

This protein is of low abundance in plant tissues.

Protocol for tonoplast membrane isolation can be found here.

application information

1: 8000 (ELISA), 1: 1000 with standard ECL (WB)

111 | 110 kDa (Raphanus sativus)

Arabidopsis thaliana, Brassica oleracea, Raphanus sativus

dicots including: Vitis vinifera, monocots including: Oryza sativa, Triticum aestivum, trees: Populus balsamifera, moss: Physcomitrella patens

no confirmed exceptions from predicted reactivity known in the moment

Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.

Manufactured by Operon Biotechnologies.

to be added when available 

application example

1 µg and 10 µg of crude membrane fraction/lane from Raphabus sativa L. Tokinashi-daikon  were separated on 12 % SDS-PAGE and blotted 1h to PVDF membrane (40 min. at 10 V using BioRad semidry transfer). Filters were blocked 1h with 5 % low-fat milk powder in TBS-T (0.05% Triton X.100). Membranes were washed 5 times with TBS-T, each time in a fresh polystyrene box and probed with anti-Ca2+ ATPase  antibodies (AS09 486, 1:1000, 1h) and secondary anti-rabbit (1:2000, 1 h). All steps were performed in RT with agitation.