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Plant/Algal cell antibodies
- Protein standards-quantitation
- Global antibodies
- Compartment markers
- Bioenergetics
- Carbohydrates
- Developmental biology
- DNA/RNA/cell cycle
- Environmental stress
- Fermentation
- Food proteins
- Hormones
- Mitochondria | Respiration
- Membrane transport system
- Nitrogen metabolism
- Photosynthesis
- Plant pathogens
- Toxins
- Tag antibodies
- Secondary antibodies/blocking
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Animal cell antibodies
- Bacterial, insect and fungal
- Carrier proteins
- Fish proteins
- Human proteins
- Immunoglobulins
- Neurosteroids/Neurobiology
- Secondary antibodies/blocking
- Secondary antibodies
Ca2+-ATPase | calmodulin-stimulated calcium-ATPase
AS09 486 | clonality: polyclonal | host: rabbit | reactivity: Arabidopsis thaliana, Brassica oleracea, Raphanus sativus
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| application information |
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| recommended dilution | 1: 8000 (ELISA), 1: 1000 with standard ECL (WB) |
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| expected | apparent MW | 111 | 110 kDa (Raphanus sativus) |
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| confirmed reactivity | Arabidopsis thaliana, Brassica oleracea, Raphanus sativus |
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| predicted reactivity | dicots including: Vitis vinifera, monocots including: Oryza sativa, Triticum aestivum, trees: Populus balsamifera, moss: Physcomitrella patens |
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| not reactive in | no confirmed exceptions from predicted reactivity known in the moment |
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| additional information | Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel. Manufactured by Operon Biotechnologies. |
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| selected references | to be added when available |
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| application example
1 µg and 10 µg of crude membrane fraction/lane from Raphabus sativa L. Tokinashi-daikon were separated on 12 % SDS-PAGE and blotted 1h to PVDF membrane (40 min. at 10 V using BioRad semidry transfer). Filters were blocked 1h with 5 % low-fat milk powder in TBS-T (0.05% Triton X.100). Membranes were washed 5 times with TBS-T, each time in a fresh polystyrene box and probed with anti-Ca2+ ATPase antibodies (AS09 486, 1:1000, 1h) and secondary anti-rabbit (1:2000, 1 h). All steps were performed in RT with agitation. |
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||| For applications or usage on species others than stated above Agrisera offers a payment-after-testing option. To learn more about this or for any questions on this product, please use the LiveChat option in the left menue bar or contact us at support@agrisera.com

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