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product information
|
| background |
|
3-hydroxybutyrate dehydrogenase (EC=1.1.1.30) is an oxidoreductase which participates in synthesis and degradation of ketone bodies and butanoate metabolism. Alternative names: NAD+-beta-hydroxybutyrate dehydrogenase, hydroxybutyrate oxidoreductase, beta-hydroxybutyrate dehydrogenase, D-beta-hydroxybutyrate dehydrogenase, D-3-hydroxybutyrate dehydrogenase, D-(-)-3-hydroxybutyrate dehydrogenase, beta-hydroxybutyric acid dehydrogenase, -D-hydroxybutyrate dehydrogenase, and beta-hydroxybutyric dehydrogenase. |
| immunogen |
|
3-hydroxybutyrate dexydrogenase isolated and purified from Rhodobacter sphaeroides |
| antibody format |
|
rabbit |
polyclonal |
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purified IgG in phosphate buffer saline, |
lyophilized |
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| quantity |
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1 ml |
for reconstitution please add 1 ml of sterile destilled water. |
|
| storage |
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store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. |
| tested applications |
|
western blot (WB), ELISA (ELISA),immunofluorescence (IF), immunohistochemistry (IHC) |
| related products |
|
AS09 562 | anti-3-hydroxybutyrate dexydrogenase AS09 564 | anti-3-hydroxybutyrate dexydrogenase (affinity purified) |
| additional information |
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Antibody is labelled with biotin using N-hydroxysuccinimidobiotin. Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition. |
application information
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| recommended dilution |
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1: 100 - 1: 1000 for techniques listed above |
| expected | apparent MW |
|
26.9 kDa |
| confirmed reactivity |
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Rhodopacter shpaeroides |
| predicted reactivity |
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Sagittula stellata, Silicibacter sp. |
| not reactive in |
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no confirmed exceptions from predicted reactivity known in the moment |
| additional information |
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Biotin/IgG protein molar ration is approximately 6.2. No foreign proteins are added. |
| selected references |
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to be added when available |
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