|
product information
|
| background |
|
ADP-glucose pyrophosphorylase (AGPase) catalyses the first committed step in the synthesis of transient starch in chloroplasts and storage starch in amyloplasts in plants (Tetlow et al., 2004). AGPase in higher plants is composed of two distinct subunits encoded by separate genes, forming an L2S2 heterotetramer. The large subunits (L) are modulators of allosteric activity, while the small subunits (S) are the catalytic subunits (Kim et al., 2007). Synonymes:ADP-glucose pyrophosphorylase, ADP-glucose synthase, AGPase B, Alpha-D-glucose-1-phosphate adenyl transferase |
| immunogen |
|
KLH-conjugated synthetic peptide derived from know sequences of small and large subunit of ADP-glucose pyrophosphorylase including Arabidopsis thaliana P55228 (small subunit), P55229, P55231 (large subunit) |
| antibody format |
|
rabbit |
polyclonal |
|
serum |
lyophilized |
|
| quantity |
|
200 µl |
for reconstitution add 200 µl, of sterile water. |
|
| storage |
|
store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. |
| tested applications |
|
western blot (WB) |
| related products |
|
other antibodies involved in carbohydrate metabolism |
| additional information |
|
Antibody is recognizing both, large and small subunit of ADGP enzyme. |
application information
|
| recommended dilution |
|
1 : 1000 with standard ECL (WB) |
| expected | apparent MW |
|
49.4 | 52 |
| confirmed reactivity |
|
Arabidopsis thaliana, Horderum vulgare, Zea mays, Chlamydomonas reinhardtii
|
| predicted reactivity |
|
dicots including: Beta vulgaris, Solanum lycopersicum, Vitis vinifera, monocots including: Oryza sativa, Triticum aestivum, algae: Chlamydomonas reinhardtii, cyanobacteria and other bacteria (chlamydiae, planctomycetes, proteobacteria, spirochetes, and verrucomicrobia). |
| not reactive in |
|
no confirmed exceptions from predicted reactivity are currently known |
| additional information |
|
to be added when available |
| selected references |
|
to be added when available |
application example 
5 µg of total protein from Arabidopsis thaliana (1), Hordeum vulgare (2), Zea mays (3), Chlamydomonas reinhardtii (4) extracted with Agrisera protein extraction buffer PEB were separated on 4-12% NuPAGE and blotted 1h to PVDF. Blots were blocked with ECL Advance Blocking Reagent for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 10 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602) diluted to 1:50 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL Advance according to the manufacturers instructions (GE Healthcare). Exposure time was 270 seconds.
||| For applications or usage on species others than stated above Agrisera offers a payment-after-testing option. To learn more about this or for any questions on this product, please use the LiveChat option in the left menue bar or contact us at support@agrisera.com
 Save as PDF
|
