Plant/Algal cell antibodies / Carbohydrates

product information

AKIN beta-2 (AKINB2) is a member of SnRK1/SNF1/AMPK family in plants. It is a regulatory subunit of the putative trimeric SNF1-related protein kinase (SnRK) comples which may play a role in a signal transduction cascade regulating gene expression and carbohydrate metabolism in higher plants.

KLH-conjugated peptide derived from Arabidopsis thaliana AKIN beta-2 Q9SCY5

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

western blot (WB)

AS09 460 | AKINB1 | SNF1-related protein kinase regulatory subunit beta-1

AS09 613 | AKIN beta-3

AS09 463 | AKIN beta gamma

to be added when available

application information

2 µg/ml

31.9 | 35 kDa

Arabidopsis thaliana

Arabidopsis thaliana

no confirmed exceptions from predicted reactivity known in the moment

to be added when available

to be added when available, antibody released in December 2009

application example

25 µg of empty vector (1), pCDNA 3.1 – HA- tagged AKIN-beta1 (2), pCDNA 3.1 – HA- tagged AKIN-beta2 (3), 50 µg of total protein from Arabidopsis thaliana leaves had been homogenized into 50 mM NaPO4 pH 7.5, 20 mM KCl, 0.5 M sucrose, 0.2 mM PMSF, 10 mM DTT and protease inhibitor cocktail (Sigma, P9599). Extracts were separared on 10%NuPage Bis-Tris Novex SDS PAGE (Invitrogen) gels followed by a transfer for 60 min to PVDF membrane. Filters were blocked for 1h with 5% low-fat milk powder in PBS-T (0.1% TWEEN 20) and probed with anti-AKINbeta-2  antibodies (2 µg/ml for 60 min at RT) followed by Protein G-HRP (Bio-Rad; 1:3 000 for 60 min at RT) in PBS-T. Antibody incubations were followed by washings in PBS-T (3 x 5 min). All washing steps were performed at RT with agitation. Signal was detected with ECL (Millipore) using CCD camera. Exposure time was 10 seconds.

Courtesy Dr. David Stapleton