Plant/Algal cell antibodies / Carbohydrates

product information

Isocitrate lyase (EC=4.1.3.1), localized in glyoxysome is an enzyme involved in mobilization of storage lipis during plant seedling growth. Catalalyzed reaction is a conversion of isocitrate to succinate and glyoxylate. Alternative names of this enzyme: isocitrase, isocitritase, isocitratase, glyoxylate-lyase, isocitrate glyoxylatelyase.

purified native ICL from Ricinus communis

store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.

western blot (WB), ELISA (ELISA)

collection of antibodies to proteins involved in carbohydrate metabolism

0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.

application information

1: 8000 (ELISA), 1: 2000 with standard ECL (WB)

64.7 | 65  kDa

Ricinus communis

dicots including: Arabidopsis thaliana, Cucumis sativus, Gossypium mexicanum, monocots including: Oryza sativa, trees: Populus balsamifera subsp. trichocarpa

no confirmed exceptions from predicted reactivity known in the moment

Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.

Manufactured by Operon Biotechnologies.

Maeshima et al. (1988). Evidence for no proteolytic processing during transport of isocitrate lyase into glyoxysomes in castor bean endosperm. Plant Cell Physiol. 29:381-384.

application example

SDS-PAGE gel electrophoresis of the purified ICL (1) and immunoselected protein (2) prepared according to Plant and Cell Physiol. 1988, 29: 381-384. Gel was stained with Coomasie brilliant blue R. Fluorogram of the immunoselected protein from the 2-day -old endosperm of castor bean labeled in vivo with 35S-methionine (3).