Plant/Algal cell antibodies / Carbohydrates

product information

SPS (sucrose phosphate synthase, EC 2.4.1.14) is the key enzyme of carbon flux into sucrose fixation in plants. It catalyzes the synthesis of sucrose-phosphate from UDP-glucose and fructose-6-phosphate predominantly in the cytosol of sucrose-source leaf tissue.

KLH-conjugated synthetic peptide derived from conserved region within plant SPS protein sequences, including Arabidopsis thaliana isoforms 1F Q94BT0, 2F, 3F and 4F. Oryza sativa Q67WN8, Solanum tuberosum Q43845

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

western blot (WB)

AS06 185 | anti-SPS sucrose phosphate antibody, maize

to be added when available

application information

1: 1000 - 1: 5000 with standard ECL (WB)

120 | 120-130 kDa (fragments of 30/90 kDa may be detected)

Arabidopsis thaliana, Hordeum vulgare, Lycopersicum esculentum, Lycopersicum penelli, Solanum tuberosum, Triticum aestivum, Zea mays

dicots including: Glycine max, Vicia faba,Solanum tuberosum,  monocots including: Oryza sativa, trees: Populus balsamifera,  Robinia pseudoacacia, moss: Physcomitrella patens

no confirmed exceptions from predicted reactivity known in the moment

Peptide used to elicit anti-SPS antibodies is perfectly conserved in all isoforms of SPS in plants.

Bölter et al. (2007) Localization of Arabidopsis NDPK2-revisited. Planta 226: 1059-1056

Whittaker et al. (2007) Sucrose phosphate synthase activity and the co-ordination of carbon partitioning during sucrose and amino acid accumulation in desiccation-tolerant leaf material of the C4 resurrection plant Sporobolus stapfianus during dehydration. J Ex. Bot. 58: 3775-3787

application example

10 µg of total leaf protein from (1) A.thaliana,(3) Zea mays and (4) Hordeum vulgare extracted with PEB (AS08 300) as well as 10 µg cytosolic protein from (2) A.thaliana were separated on 4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1.5h (30V) to nitrocellulose. Filters were blocked 1h with 2% low-fat milk powder in TBS-T (0.1% TWEEN 20) and probed with anti-SPS (AS03 035A, 1:2000, 1h) and secondary anti-rabbit (1:20000, 1 h) antibody (HRP conjugated, Abcam) in TBS-T containing 2% low fat milk powder.  Antibody incubations where followed by washings in TBS-T (15, +5, +5, +5 min). All steps were performed at RT with agitation. Signal was detected with standard ECL (GE Healthcare) using a Fuji LAS-3000 CCD (90s, high sensitivity).