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Plant/Algal cell antibodies
- Protein standards-quantitation
- Global antibodies
- Compartment markers
- Bioenergetics
- Carbohydrates
- Developmental biology
- DNA/RNA/cell cycle
- Environmental stress
- Fermentation
- Food proteins
- Hormones
- Mitochondria | Respiration
- Membrane transport system
- Nitrogen metabolism
- Photosynthesis
- Plant pathogens
- Toxins
- Tag antibodies
- Secondary antibodies/blocking
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Animal cell antibodies
- Bacterial, insect and fungal
- Carrier proteins
- Fish proteins
- Human proteins
- Immunoglobulins
- Neurosteroids/Neurobiology
- Secondary antibodies/blocking
- Secondary antibodies
SPS | sucrose phosphate synthase, global
AS03 035A | affinity purified | clonality: polyclonal | host: rabbit | reactivity: [global antibody] for di and monocots | cellular [compartment marker] of cytoplasm for photosynthetic tissues.
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| application information |
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| recommended dilution | 1: 1000 - 1: 5000 with standard ECL (WB) |
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| expected | apparent MW | 120 | 120-130 kDa (fragments of 30/90 kDa may be detected) |
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| confirmed reactivity | Arabidopsis thaliana, Colobanthus quitensis Kunt Bartl, Hordeum vulgare, Lycopersicum esculentum, Lycopersicum penelli, Solanum tuberosum, Triticum aestivum, Pinus strobus, Zea mays |
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| predicted reactivity | dicots including: Glycine max, Vicia faba,Solanum tuberosum, monocots including: Oryza sativa, trees: Populus balsamifera, Robinia pseudoacacia, moss: Physcomitrella patens |
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| not reactive in | no confirmed exceptions from predicted reactivity known in the moment |
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| additional information | Peptide used to elicit anti-SPS antibodies is perfectly conserved in all isoforms of SPS in plants. |
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| selected references | Bölter et al. (2007) Localization of Arabidopsis NDPK2-revisited. Planta 226: 1059-1056 Whittaker et al. (2007) Sucrose phosphate synthase activity and the co-ordination of carbon partitioning during sucrose and amino acid accumulation in desiccation-tolerant leaf material of the C4 resurrection plant Sporobolus stapfianus during dehydration. J Ex. Bot. 58: 3775-3787 |
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application example 10 µg of total leaf protein from (1) A.thaliana,(3) Zea mays and (4) Hordeum vulgare extracted with PEB (AS08 300) as well as 10 µg cytosolic protein from (2) A.thaliana were separated on 4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1.5h (30V) to nitrocellulose. Filters were blocked 1h with 2% low-fat milk powder in TBS-T (0.1% TWEEN 20) and probed with anti-SPS (AS03 035A, 1:2000, 1h) and secondary anti-rabbit (1:20000, 1 h) antibody (HRP conjugated, Abcam) in TBS-T containing 2% low fat milk powder. Antibody incubations where followed by washings in TBS-T (15, +5, +5, +5 min). All steps were performed at RT with agitation. Signal was detected with standard ECL (GE Healthcare) using a Fuji LAS-3000 CCD (90s, high sensitivity). |
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||| For applications or usage on species others than stated above Agrisera offers a payment-after-testing option. To learn more about this or for any questions on this product, please use the LiveChat option in the left menue bar or contact us at support@agrisera.com

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