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product information
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| background |
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COP1 (E3 ubiquitin-protein ligase COP1) is involved in phytochrome signaling pathway and acts as a repressor of photomorphogenesis and as an activator of etiolation in the darkness. COP1 is repressing photomorphogenesis in darkness by mediating ubiquitination followed by a subsequenct proteasomal degradation of light-induced transcription factors (HY5, HYH and LAF1). This protein is localized in nucleus in the darkness and upon illumination is gradually transferred into cytoplasm.
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| immunogen |
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KLH- conjugated synthetic peptide derived from known COP1 sequences including Arabidopsis thaliana P43254 |
| antibody format |
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rabbit |
polyclonal |
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affinity purified |
lyophilized |
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| quantity |
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200 µg |
- for reconstitution add 200 µl of sterile water |
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| storage |
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store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. |
| tested applications |
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Western blot (WB) |
| related products |
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AS08 307 | Anti-UBQ11 | Ubiquitin rabbit antibody
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| additional information |
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to be added when available
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application information
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| recommended dilution |
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1: 2000 with standard (ECL) |
| expected | apparent MW |
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76 kDa (Arabidopsis thaliana) |
| confirmed reactivity |
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Vicia faba
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| predicted reactivity |
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dicots including: Arabidopsis thaliana, Brassica napus, Pisum sativum, Ricinus communis, Solanum lycopersicum, monocots including: Oryza sativa, moss: Physcomitrella patens
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| not reactive in |
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no confirmed exceptions from predicted reactivity known in the moment |
| additional information |
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to be added when available |
| selected references |
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to be added when available, antibody released in March 2011 |
application example 
3.5 µg of total protein from 4-day old Vicia faba roots, was separated on 12 % SDS-PAGE and blotted 50 min to PVDF membrane. Blots were blocked immediately following transfer in MTBS-T (5 % milk) for 30 min. at RT with agitation. Blots were incubated in the primary antibody at a dilution of 1: 2000 for 1 h ar RT with agitation. The antibody solution ws decanted and the blot was rinsed briefly twice, following by a 3 times was for 3 min. in TBS-T at RT with agitation. Blots were incubated with secondary antibody (anti-rabbit IgG, HRP conjugated from Agrisera, AS09 602) in 1: 20 000 dilution for 30 min. at RT with agitation. The blots were washed as above and developed for 5 min. with ECL Advance detection reagent (GE Healthcare) according to the manufactures instructions. Exposure time was 60 seconds. Courtesy of Dr. Dorota Rybaczek
||| For applications or usage on species others than stated above Agrisera offers a payment-after-testing option. To learn more about this or for any questions on this product, please use LiveChat option in the left menu or contact us at support@agrisera.com
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