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Plant/Algal cell antibodies / Chlamydomonas reinhardtii


AtpA | alpha subunit of ATP synthase

Art no: AS08 304
Price: 304
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product information

background  

ATP synthase is the universal enzyme that synthesizes ATP from ADP and phosphate using the energy stored in a transmembrane ion gradient. AtpA is the largest subunit of the membrane-extrinsic ATP synthase subcomplex.

immunogen  

recombinant maize chloroplas P05022

antibody format  

rabbit;

polyclonal;

serum;

lyophilized

quantity  

100 µl

- for reconstitution add 100 µl of sterile water

storage  

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

tested applications  

Western blot (WB)

related products  

AS03 030 | anti-ATP synthase subunit beta hen antibody

AS08 312 | anti-ATP synthase subunit gamma antibody

AS05 071 | anti-ATP synthase subunit c antibody

AS08 370 | anti-ATP synthase whole enzyme

additional information  

sequence of protein used for eliciting this antibody is also conserved in Arabidopsis thaliana AtpA P56757

application information

recommended dilution  

1: 10 000 with standard ECL (WB)

expected | apparent MW  

55 kDa

confirmed reactivity  

Arabidopsis thaliana, Zea mays

predicted reactivity  

established to react with dicot and monocot chloroplast AtpA; may cross-react with mitochondrial AtpA; algae, cyanobacteria

not reactive in  

no confirmed exceptions from predicted reactivity known in the moment

additional information  

to be added when available

selected references  

to be added when available


application example

 

Western blot
 
Lane 1: Arabidopsis thaliana total leaf protein extract;
Lane 2: Zea mays total leaf protein extract
5 µg of total protein were loaded per each lane.
 
Experimental conditions:
 
Proteins were separated in a 12% or 5-15% gradient gel following by a transfer to nitrocellulose membrane. Membrane was blocked with TBST + 4% non-fat dried milk, 20 min following by three washes in TBST. Incubation time with primary and secondary antibodies was 1 hr primary, 30 min for secondary antibodies. Manufacturer of secondary antibodies: Bio-Rad
AS08 304 western blot picture.jpg
 

 


||| For applications or usage on species others than stated above Agrisera offers a payment-after-testing option. To learn more about this or for any questions on this product, please use LiveChat option in the left menu or contact us at support@agrisera.com



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