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CDJ1 | chloroplast DnaJ homolog 1

320 €

AS06 179  |  clonality: polyclonal  | host: rabbit  |  reactivity:Chlamydomonas reinhardtii

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Item No:
AS06 179

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product information
background  

CDJ1 is one of three zinc-finger containing J domain proteins of Chlamydomonas reinhardtii. This protein is found in chloroplast. In spite that CDJ1 gene is heat shock inducible it does not reflect in increased CDJ1 protein levels. CDJ1 forms complexes with plastidic HSP90C,HSP70B and CGE1 and is a new cohort protein of the chloroplast multi-chaperone complex.DnaJ/Hsp40 (heat shock protein 40) proteins are highly conserved and play crucial roles in protein translation, folding, unfolding, translocation, and degradation.

immunogen  

fusion protein encoding region for the Chlamydomonas rerinhardtii N-terminal part of CDJ1 Q66YD3, overexpressed in E.coli

antibody format  

rabbit

polyclonal

serum

lyophilized

quantity  

100 µl

for reconstitution add 100 µl of sterile water.

storage  

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

tested applications  

western blot (WB) immunoprecipitation (IP)

related products  

collection of antibodies to other heat shock proteins

collection of antibodies to Chlamydomonas reinhardtii

collection of antibodies to chloroplast proteins

additional information  

to be added when available

application information
recommended dilution  

1. 3000 with standard ECL (WB)

expected | apparent MW  

40 | 40 kDa

confirmed reactivity  

Chlamydomonas reinhardtii

predicted reactivity  

Ostreococcus sp.

not reactive in  

higher plants

additional information  

western blot has been done on 10-50 µg load per well; immunoprecipitation has been done on 5-50 µl

selected references  

Willmund et al (2008). The chloroplast DnaJ homolog CDJ1 of Chlamydomonas reinhardtii is part of a multichaperone complex containing HSP70B, CGE1, and HSP90C. Plant Physiol. 148: 2070-2082.



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