Plant/Algal cell antibodies / Chlamydomonas reinhardtii

product information

Iron-hydrogenase HydA2 is catalyzing reversible oxidation of molecular hydrogen. In Chlamydomonas the protein is present in low levels of 1 µg/liter of culture.Synonyme: HYD1/1

recombinant, full length Chlamydomonas reinhardtii HydA-2 Q8VZZ0

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

western blot (WB) 

AS09 600 | anti-HydA | iron-hydrogenase HydA2 rabbit antibodies

collection of antibodies to Chlamydomonas reinhardtii

In Chlamydomonas HydA is present in low levels of 1 µg/liter of culture. Therefore, an induction of cells by anaerobic adaptation or sulfur depravation (10 x higher amount than with anaerobic adaptation) is necessary for successful detection using this antibody. Methods of HydA induction are described in Hemschemeier et al. 2009. 

to detect HydA1/A2 in Chlamydomonas extracts amount loaded per well corresponds to 2 µg of chlorophyll for sulfur deprived cells, where relatively much HydA1 is synthesized or corresponds to 2-4 µg of artificially anaerobic induced cultures, where the HydA1 protein level is lower. This antibody is recognizing 1 ng of recombinant HydA protein.

application information

1 : 5000 with standard ECL (WB)

53.7 | 48 kDa (after transit peptide is cleaved)

Chlamydomonas reinhardtii

Ostreococcus sp.

no confirmed exceptions from predicted reactivity known in the moment

HydA1 (497aa) has a calculated MW of 53.1 kDa, but this is including the signal peptide, which gets cleaved off. The protein without TP has a calculated MW of 47.5 kDa and runs according to its size at about 48 kDa.
HydA2 (505aa) has a calculated MW of 53.7 kDa, but this is including the signal peptide, which gets cleaved off. The protein without TP can only be estimated, since the cleavage site is known only from in silico analysis. It has a calculated MW of 47.3 kDa and should run in the gel also according to its size.

Magneschi et al. (2012). A Mutant in the ADH1 Gene of Chlamydomonas reinhardtii Elicits Metabolic
2 Restructuring during Anaerobiosis. Plan Physiol. January 23 (ahead of print).

Lecier et al. (2011). Functional analysis of hydrogen photoproduction in respiratory-deficient mutants of Chlamydomonas reinhardtii. International Journal of Hydrogen Energy

application example

50ng of purified protein (HydA1 and HydA2) were separated on 10% SDS-PAGE and blotted 25 min to PVDF membrane. Filters were blocked 1h with 3% low-fat milk powder in PBS-T (0.1% TWEEN 20) and probed with anti-HydA1/2 (AS09 514, 1:5000, over night at 4°C) and secondary anti-rabbit (1:10 000, 1 h) antibody (HRP conjugated, manufacture Pierce) in PBS-T containing 3% low fat milk powder. Antibody incubations were followed by washings in PBS-T (10, +10min and PBS (+5, +5 min). All washing steps were performed at RT with agitation. Signal was detected with ECL (Millipore) using CCD camer. Exposure time was 20 min.

The heterolog expressed proteins have both calculated MWs of 51 kDa (due to the tag) and run according to their size.

Courtesy Dr. Thomas Happe