Plant/Algal cell antibodies / Chlamydomonas reinhardtii

product information

Cysteine (a non-essential amino acid) is a building block of antioxidant glutathione. With a thiol side chain, cysteine is an important structural and functional component of many proteins and enzymes.

synthetic L-Cysteine conjugated to protein carrier (PC)

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

immunocytochemistry (ICC)

to be added when available

Compounds                                              Cross-reactivity ratio (a)

L-Cysteine-G-(Pc)                                                1
DL-Homocysteine-G-(Pc)                                    1/10
L-Methionine-G-(Pc)                                           1/>1,000
L-Glutathion-G-(Pc)                                            1/>1,000
L-Cysteic acid-G-(Pc)                                         1/>1,000
L-Serine-G-(Pc)                                                 1/>1,000
Free L-Cysteine                                                 1/>10,000

(a) : L-Cysteine-G-(Pc) concentration/unconjugated or conjugated close-related compounds concentration at half displacement ; G =Glutaraldehyde. Cross-reactivity ratios were evaluated by competitive ELISA: a ratio of 1/1000 means that 1000 times higher concentration of immunogen is needed to switch off the specific signal by half.

application information

1: 1000 - 1: 5 000 (IHC)

n. a.

L-cysteine

L-cysteine

no confirmed exceptions from predicted reactivity known in the moment

example of immunohistochemical protocol can be found here.

Free cysteine will not attach to polystyrene ELISA plates. BSA-conjugated cysteine has to be used for this purpose.

Zechmann et al. (2006) Immunocytochemical localization of glutathione precursors in plant cells. J Electron Microsc (Tokyo), 55(3):173-81; Zechmann et al. (2007) Virus-induced changes in the subcellular distribution of glutathione precursors in Cucurbita pepo (L.). Plant Biol (Stuttg), 9(3):427-34