Plant/Algal cell antibodies / Chlamydomonas reinhardtii

product information

Plastocyanin is a “blue” copper protein which catalyzes electron transfer between the cytochrome b6 .f complex and P-700, the reaction center of photosystem I. Plastocyanin is a nuclear encoded polypeptide in all eukaryotic photosynthetic organisms where it has been studied. It is synthesized as a pre-protein of approximate molecular weight 17,000, imported post-translationally into chloroplasts, and processed to its mature form of approximate molecular weight 10,500 within the plastid.

GST fusion to Pre-apoplastocyanin of Chlamydomonas reinhartii  P18068

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

western blot (WB)

AS06 141 | anti-plastocyanin

Collection of antibodies to Chlamydomonas proteins

to be added when available

application information

1: 1000 with alkaline phosphatase (WB)

17 kDa (precursor)

Chlamydomonas reinhardtii

Chlorella fusca Pediastrum boryanum

no confirmed exceptions from predicted reactivity known in the moment

This antibody is recognizing pre-apoplatosyanin 17 kDa precursor and a mature protein. There is a slight cross-reactivity with cytochreom c6 in extract from Chlamydomonas reinhardtii cells grown under conditions of cooper deficiency.

Li et al.  (1996).  Molecular genetic analysis of plastocyanin biosynthesis in Chlamydomonas reinhardtii.. J. Biol. Chem. 271:31283-31289