Plant/Algal cell antibodies / Chlamydomonas reinhardtii

product information

PsaC is a conserved, chloroplast-encoded, Fe-S binding protein of approximately 10kDa, present in all known Photosystem I complexes. It is located on the stromal side of the thylacoid membranes. PsaC coordinates the Fe–S clusters FA and FB through two cysteine-rich domains.

KLH-conjugated synthetic peptide conserved in all known PsaC proteins including Arabidopsis thaliana AtCg01060, Hordeum vulgare P69416, Oryza sativa P0C360, Chlamydomonas reinhardtii Q00914, Synechococcus elongatus Q31QV2

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

western blot (WB)

AS10 939 | PsaC | PSI-C core subunit of photosystem I  antibody, 200 ul

AS04 042S | PsaC protein standard for quantitation or positive control

collection of antibodies to PSI proteins

Peptide target used to elicit this antibody is well conserved in all photoautotrophs except some cyanobacteria, some red algae and Cyanophora paradoxa, which contain a conserved substitution of a valine to an isoleucine. The  performance of the antibodies has been confirmed against taxa containing both the valine and isoleucine variants.

More information about quantitative western blot using PsaC antibodies can be found here.

application information

1: 1000 with ECL (WB)

9 kDa

Arabidopsis thaliana, Horderum vulgare, Spinacia oleracea, Synechococcus PCC 7942, Cyanophora paradoxa, Heterosigma akashiwo, Thalassiosira pseudonan, Euglena gracilis, Micromonas pusilla, Chlamydomonas reinhardtii, Porphyra sp., Gonyaulax polyedra, Emiliania huxleyi, Thalassiosira pseudonana

dicots including Glycine max, Nicotiana tabacum, Spinacia oleracea, and monocots, Physcomitrella patens, algae and cyanobacteria, Prochlorococcus sp. (surface and a deep water ecotype)

no confirmed exceptions from predicted reactivity known in the moment

In some species minor cross reactions with some larger proteins are seen. These may contain related iron-sulfur binding motifs. Therefore size verification of the reacting band is required. Due to the small size of the protein, care should be taken to differentiate between chemiluminescent signal from PsaC and non-specific signals from chlotophylls or lipids if pigment is retained near the bottom of the blot.