Plant/Algal cell antibodies / Chlamydomonas reinhardtii

product information

The 22 kDa PsbS protein of photosystem II functions in the regulation of photosynthetic light harvesting. Along with a low thylakoid lumen pH and the presence of de-epoxidized xanthophylls, PsbS is necessary for photoprotective thermal dissipation of excess absorbed light energy in plants, measured as non-photochemical quenching of chlorophyll fluorescence.

KLH-conjugated synthetic peptide derived from available di and monocot PsbS sequences, including Arabidopsis thaliana (At1g44575)

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

western blot (WB)

AS03 032 | anti-PsbS hen antibody

PSII  available antibodies against Photosystem II proteins

to be added when available

application information

1 : 2000 with standar ECL (WB)

28 | 22 kDa for Arabidopsis thaliana

dicots including: Arabidopsis thaliana, Ricinus communis, Spinacia oleracea, monocots including: Hordeum vulgare, Zea mays

dicots including: Nicotiana tabacum, Solanum lycopersicum, Vitis vinifera, monocots including: Oryza sativa, trees: Picea sitchensis, Populus balsamifera, moss: Physcomitrella patens, algae: Chlamydomonas reinhardtii

no confirmed exceptions from predicted reactivity known in the moment

to be added when available

Zienkiewicz et al. (2011). High light stimulates Deg1-dependent cleavage of the minor
LHCII antenna proteins CP26 and CP29 and the PsbS protein in Arabidopsis thaliana. Planta Aug 30.

application example

5 µg of total extract from (1) Arabidopsis thaliana leaf,  (2) Spinacia oleracea  (3) Hordeum vulgare (4) Zea mays extracted with PEB (AS08 300) were separated on 4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to PVDF. Blots were blocked immediately following transfer in 2% ECL Advance blocking reagent (GE Healthcare) in 20 mM Tris, 137 mM sodium chloride pH 7.6 with 0.1% (v/v) Tween-20 (TBS-T) for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 10 000 for 1h at room temperature with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Abcam) diluted to 1:50 000 in 2% ECL Advance blocking solution for 1h at room temperature with agitation. The blots were washed as above and developed for 5 min with ECL Advance detection reagent according to the manufacturers instructions. Images of the blots were obtained using a CCD imager (FluorSMax, Bio-Rad) and Quantity One software (Bio-Rad). Exposure time was 30 seconds. 

application example

15 µg of total extract from (1) Arabidopsis thaliana leaf,  (2) Arabidopsis thaliana thylakoid fraction  (3) Arabidopsis thaliana NPQ4 mutant thylakoids (4) Arabidopsis thaliana PsbS overexpression mutant   were separated on 4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to PVDF. Blots were blocked immediately following transfer in 2% ECL Advance blocking reagent (GE Healthcare) in 20 mM Tris, 137 mM sodium chloride pH 7.6 with 0.1% (v/v) Tween-20 (TBS-T) for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 20 000 for 1h at room temperature with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Abcam) diluted to 1:50 000 in 2% ECL Advance blocking solution for 1h at room temperature with agitation. The blots were washed as above and developed for 5 min with ECL Advance detection reagent according to the manufacturers instructions. Images of the blots were obtained using a CCD imager (FluorSMax, Bio-Rad) and Quantity One software (Bio-Rad). Exposure time was 30 seconds.