|
product information
|
| background |
|
Ascorbate oxidase (AO) is an apoplastic enzyme involved in metabolism of plant ascorbate (AA). Ascorbate (AA) plays a key role in defense against oxidative stress and is particularly abundant in photosynthetic tissues. Over 90% of the ascorbate is localized in the cytoplasm, but a substantial proportion is exported to the apoplast. |
| immunogen |
|
ascorbate oxidase purified from Cucurbita sp. |
| antibody format |
|
rabbit |
polyclonal |
|
total IgG in PBS pH 7.4 |
lyophilized |
|
| quantity |
|
100 µl |
for reconstitution add 100 µl of sterile water. |
|
| storage |
|
store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. |
| tested applications |
|
western blot (WB) |
| related products |
|
AS09 383 | anti-ascorbate oxidase biotinylated antibody |
| additional information |
|
IgG concentration is 4 µg/µl |
application information
|
| recommended dilution |
|
1:2 000 with standard ECL (WB) |
| expected | apparent MW |
|
61 | 45 kDa (Cucurbita pepo) |
| confirmed reactivity |
|
Cucurbita sp., Nicotiana tabacum, Solanum tuberosum, Zea mays |
| predicted reactivity |
|
dicots including Glycine max, monocots including Oryza sativa |
| not reactive in |
|
no confirmed exceptions from predicted reactivity known in the moment |
| additional information |
|
antibody can be used as a marker of apoplast |
| selected references |
|
Kielbowicz et al. (2008) The organ-dependent abundance of a Solanum lipid transfer protein is up-regulated upon osmotic constraints and associated with cold acclimation ability. J. Expo Bot. 59:2191-2203. |
||| For applications or usage on species others than stated as confirmed above Agrisera offers a payment-after-testing option. To learn more about this or for any questions on this product, please use the LiveChat option in the left menue bar or contact us at support@agrisera.com
 Save as PDF
|
