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Cyt c6 | thylakoid lumen cytchrome c6 protein

320 €

AS06 202 | clonality: polyclonal | host: rabbit | reactivity: Chlamydomonas reinhardtii | cellular [compartment marker] of thylakoid lumen

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Item No:
AS06 202

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product information
background  

Cytochrome c6 is known as a redox carrier of the thylakoid lumen of cyanobacteria and some eukaryotic algae that can substitute for plastocyanin in electron transfer.
Cytochrome c6 is situated between the two membrane-bound complexes cytochrome b6f and photosystem I (PSI) in oxygenic photosynthesis..
Cytochrome c6 (cyt c6) in Chlamydomonas reinhardtii is synthesized only under conditions of copper deficiency when plastocyanin cannot be synthesized.

immunogen  

GST fusion of recombinant Chlamydomonas reinhardtii cytochrome c6 A8J9G6

antibody format  

rabbit

polyclonal

serum

lyophilized

quantity  

200 µl

for reconstitution add 200 µl of sterile water.

storage  

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

tested applications  

western blot (WB)

related products  

AS06 122 | anti-CRD1 | cyanobacterial homolog of plant CHL27 cyclase, marker of thylakoid and envelope membranes


AS05 084 | anti-PsbA rabbit antibody, marker of thylakoid membrane


Collection of antibodies to Chlamydomonas proteins

additional information  

cellular [compartment marker] of thylakoid lumen for Chlamydomonas reinhardtii

application information
recommended dilution  

1 : 1 000 with alkaline phosphatase (WB)

expected | apparent MW  

15 kDa

confirmed reactivity  

Chlamydomonas reinhardtii

predicted reactivity  

Chlorella vulgaris, Pediastrum duplex, Cladophora glomerata

not reactive in  

no confirmed exceptions from predicted reactivity known in the moment

additional information  

Note: antibody will work better with nitrocellulose than with PVDF membrane. Recommended load per well min. 6 µg. Primary antibody incubation needs to be done over night. For detection image please refer to the reference below

selected references  

Merchant et al. (1998) Copper-Responsive Gene Expression in Photosynthetic Microorganisms. Methods in Enzymology 297:263-279.



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