Plant/Algal cell antibodies / Compartment markers / Chloroplast

product information

Plastoglobules are lipoprotein particles which can be found in chloroplasts. They are generally believed to have a function of lipid storage. Recent data suggest that plastoglobules can be also metabolically active, taking part in tocopherol synthesis and likely other pathways. Immunogen: Alternative name AtPap1, fibrillin-1, probable plastid-lipid-associated protein 1.

recombinant Arabidopsis thaliana PGL35 protein  O81439

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

western blot (WB); immunocytochemistry (IC)

n.a.

cellular [compartment marker] of chloroplast plastoglobules

For IC samples were embedded in Lowicryl HM20 and sectioned into 100-nm-thick sections and placed on Formvar-coated gold slot grids. The sections were blocked for 20 min with a 5% (w/v) solution of nonfat milk in TBS plus 0.1%Tween 20 (TBST). Anti-PGL antibodies were diluted 1:20 in a solution of 2.5% nonfat milk in TBST at room temperature for 1 h. The sections were rinsed in a stream of TBS plus 0.5% Tween 20 and then transferred to the secondary antibody (anti-rabbit IgG 1:20 in TBST) conjugated to 10-nm gold particles for 1 h. images of localization can be found in Austin et al. (2006).

application information

1: 1000 - 1: 3000 with standard ECL (WB), 1: 20 (IC)

35 kDa

Arabidopsis thaliana

Brassica napus, Brassica campestris

no confirmed exceptions from predicted reactivity known in the moment

AtPGL35 is highly similar to Pisum sativum PG1

Vidi et al. (2006) Tocopherol Cyclase (VTE1) Localization and Vitamin E Accumulation in Chloroplast Plastoglobule Lipoprotein Particles. J. Biol. Chem. 281: 11225-11234. Austin et al. (2006) Plastoglobules Are Lipoprotein Subcompartments of the Chloroplast That Are Permanently Coupled to Thylakoid Membranes and Contain Biosynthetic Enzymes. Plant Cell 18:1693-1703.